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  4. Mouse Thyroid-Peroxidase,TPO ELISA Kit

Mouse Thyroid-Peroxidase,TPO ELISA Kit

  • 中文名稱：
    小鼠甲狀腺過氧化物酶(TPO)酶聯免疫試劑盒
  • 貨號：
    CSB-E08353m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠甲狀腺過氧化物酶(TPO)酶聯免疫試劑盒（CSB-E08353m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TPO含量。TPO（血小板生成素）是刺激巨核細胞生長及分化的內源性細胞因子。它作用于血小板生成各階段，促進巨核祖細胞增殖和分化，增加血小板生成。其研究機制聚焦于明確信號傳導通路等，對治療血小板減少癥等血液疾病意義重大。試劑盒檢測范圍為2.5 ng/mL-40 ng/mL，該產品特別適用于甲狀腺功能調控機制研究、自身免疫性甲狀腺疾病模型建立、藥物或環(huán)境因素對甲狀腺代謝影響的評估等場景，為探索甲狀腺相關分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    2.5 ng/mL-40 ng/mL
  • 靈敏度：
    2.5 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 90    
    Range % 86-103    
    1:2 Average % 89    
    Range % 82-97    
    1:4 Average % 95    
    Range % 87-100    
    1:8 Average % 90    
    Range % 83-99    
  • 回收率：
    The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 94 87-102    
    EDTA plasma (n=4) 95 90-103    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    40 0.272 0.263 0.268      
    20 0.525 0.530 0.528      
    10 0.894 0.874 0.884      
    5 1.334 1.377 1.356      
    2.5 2.554 2.528 2.541      
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • 基因功能參考文獻：
    1. Lnk Deficiency Leads to TPO-Mediated Osteoclastogenesis and Increased Bone Mass Phenotype PMID: 28067429
    2. Hearing impairment in hypothyroid dwarf mice caused by mutations of the thyroid peroxidase gene. PMID: 24297261
    3. A novel hypothyroid dwarfism is due to the missense mutation Arg479Cys of the thyroid peroxidase gene in the mouse. PMID: 16762971
  • 亞細胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Peroxidase family, XPO subfamily
  • 數據庫鏈接：

    KEGG: mmu:22018

    STRING: 10090.ENSMUSP00000021005

    UniGene: Mm.4991