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  4. Mouse Tumor necrosis factor ligand superfamily member 9(TNFSF9) ELISA kit

Mouse Tumor necrosis factor ligand superfamily member 9(TNFSF9) ELISA kit

  • 中文名稱：
    小鼠腫瘤壞死因子配體超家族成員9(TNFSF9)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL023997MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠腫瘤壞死因子配體超家族成員9(TNFSF9)酶聯(lián)免疫試劑盒（CSB-EL023997MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNFSF9含量。TNFSF9 即腫瘤壞死因子超家族成員 9，也叫 4-1BBL ，是一種重要的免疫靶點。它可與 4-1BB 結(jié)合激活下游信號通路，增強(qiáng) T 細(xì)胞、NK 細(xì)胞活性和增殖能力，在腫瘤免疫治療領(lǐng)域是研究熱點，有望開發(fā)出更有效的抗癌療法。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，本試劑盒適用于自身免疫性疾病模型、腫瘤免疫治療機(jī)制研究、小鼠感染性疾病模型、腫瘤移植模型或藥物干預(yù)實驗中的分子水平動態(tài)監(jiān)測，可用于免疫通路研究、藥物靶點驗證及病理機(jī)制探索，支持科研人員高效開展體外樣本分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Tnfsf9; Cd137l; Cd157l; Ly63l; Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFSF9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    92

    Range %

    85-98

    1:2

    Average %

    96

    Range %

    89-103

    1:4

    Average %

    89

    Range %

    84-95

    1:8

    Average %

    92

    Range %

    86-98

  • 回收率：

    The recovery of mouse TNFSF9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    86

    82-91

    EDTA plasma (n=4)

    93

    89-98

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1500

    2.049

    2.134

    2.091

    1.968

    750

    1.452

    1.389

    1.420

    1.297

    375

    0.900

    0.928

    0.914

    0.790

    187.5

    0.571

    0.609

    0.590

    0.466

    94

    0.330

    0.321

    0.326

    0.202

    47

    0.231

    0.243

    0.237

    0.113

    23.5

    0.178

    0.184

    0.181

    0.058

    0

    0.122

    0.125

    0.124

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

Can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.

A:
Thanks for your inquiry!
CSB-EL023997MO
This kit is designed against extracellular domain. Pls refer to: https://www.uniprot.org/uniprot/P41274.
Capture antibody is goat polyclonal antibody and detection antibody is goat polyclonal antibody.
Immunogen is 293 cell-derived recombinant protein.
Standard is 293 cell-derived recombinant protein.
Q:

“Regarding the cncentration of sulfuric acid in this MSDS.
Is 4N sulfuric acid 2mol/l?
What is the meaning of □ of 1□ x 10ml?”

A:
Yes 4N sulfuric acid is 2mol/L. Both units are ok.
The volume of sulfuric acid in the 96T kit is 10mL.
Q:

Per customers request, please provide the following information regarding Mouse Tumor necrosis factor ligand superfamily member 9, TNFSF9 ELISA Kit (CSB-EL023997MO)
1)The active ingredient of the stop solution and its concentration ?
2)The type, concentration, and state (liquid/lyophilized) of the preservatives used ?
3) If the components are lyophilized, what is the concentration prior to reconstitution (i.e. mass of preservative used/ total mass of lyophilized vial contents)?
4)Expression host of the standards ?
5)Which components have H202 with the concentration of H202 in each, if any?
6) Are there any components that contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) in any of the components in this kit?
7) If yes, which components contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) and the concentration?

A:
Thanks for your inquiry!
CSB-EL023997MO
Standard：293 cell-derived recombinant protein（The preservatives in the standard is liquid proclin 300(1:3000)).
The standard is lyophilized, and the concentration after reconstitution is 1500pg/mL.
Stop solution is 4N sulfuric acid. ( There is no preservatives in it.)
This kit doesn't contain H202（hydrogen peroxide） & Urea hydrogen peroxide (Carbamide peroxide).
Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 功能：
    Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
  • 基因功能參考文獻(xiàn)：
    1. this study shows that intrinsic 4-1BB signals are indispensable for the establishment of an influenza-specific tissue-resident memory CD8 T-cell population in the lung PMID: 28051085
    2. 4-1BBL can restrain effector T cell development, creating a more favorable regulatory T cell to effector cell balance under tolerogenic conditions, and this may be particularly active in mucosal barrier tissues PMID: 25404362
    3. These results demonstrate that 4-1BBL-engineered DCs can improve CIKs cytotoxicity against prostate cancer cells. PMID: 25572476
    4. Data indicate that CD137L deficient mice displayed a variety of immunological dysfunctions. PMID: 24091276
    5. CD137-expressing CD4+ T cells in the bone marrow engage CD137L on hematopoietic progenitor cells, and this CD137L signaling biases hematopoiesis towards myelopoiesis during aging. PMID: 23945137
    6. Results suggest that CD137L reverse signaling exerts a pro-apoptotic effect by suppressing integrin-mediated survival signals in neural stem cells. PMID: 23925549
    7. A VCAM-1-positive stromal cell is a plausible candidate for the radioresistant cell that provides 4-1BB ligand to sustain memory CD8-positive T cells. PMID: 22886791
    8. The CD137L induces tyrosine phosphorylation as well as the up-regulation of M-CSF, IL-1beta and TN-C expressions by knockdown of TMEM126A. PMID: 22885069
    9. 4-1BB signaling negatively modulate Treg cells by two distinct mechanisms: i) inhibiting the conversion of CD4(+)FoxP3(-) T cells into iTreg cells and ii) endowing Teff cells refractory to inhibition by Treg cells. PMID: 22870329
    10. The CD137L reverse signaling pathway in epithelial cells may represent a good target for blocking the initial stage of inflammatory diseases, including renal ischemia-reperfusion injury. PMID: 22160719
    11. Reverse signals initiated by CD137L negatively modulate certain immune functions of thioglycollate-elicited peritoneal macrophages PMID: 21184130
    12. Mice inoculated with H22 tumor cells expressing B7-1, B7-2 and 4-1BBL developed a strong cytotoxic T lymphocyte response and long-term immunity against wild-type tumor, suggesting a synergistic effect between the B7 and 4-1BBL costimulatory pathways PMID: 20563597
    13. Cocultures of Natural killer (NK) cells with CD137L transfectants confirmed that human CD137 inhibits NK-cell reactivity, while activating signals were transduced by its counterpart on NK cells in mice. PMID: 20008791
    14. A role for 4-1BB ligand in dendritic cell activation PMID: 11867564
    15. In a murine model, herpetic stromal keratitis is inhibited by blockade of 4-1BBL/4-1BB interactions with monoclonal antibodies to 4-1BBL or by deletion of 4-1BB. PMID: 12847221
    16. involvement of 4-1BB in CD4(+) Th1 cell responses by regulating the clonal expansion and survival of CD4(+) T cells as seen in CD8(+) T cells PMID: 14749528
    17. Characterization of the role of 4-1BBL in activation and recall response of CD4 T cells vs CD8 T cells. PMID: 14991604
    18. demonstrate that suppression of experimental autoimmune uveoretinitis results from antigen-driven, 4-1BB-mediated expansion of novel CD11c+CD8+ T cells that suppress antigen-specific CD4+ T cells via an indole 2,3-dioxygenase-dependent mechanism PMID: 16899371
    19. indicate that the CD28 costimulatory pathway plays a major role in the alloimmune response and that 4-1BB signals are dependent upon CD28 signals PMID: 17202836
    20. IL-13 was coinduced following 4-1BB triggering to maintain the Th1/2 balance of immune response. PMID: 17389581
    21. Thus, long-term inhibition of the 4-1BB pathway reduces cardiac damage, remodeling, and inflammation during viral myocarditis. PMID: 17468777
    22. CD137 ligand plays a role in allergic asthma. PMID: 17845419
    23. CD80 and 4-1BBL induce auto- and transcostimulation in tumor cells PMID: 18026115
    24. deliver new insights into the multiple effects of reverse signaling of CD137L in human DC during the initiation of an adaptive immune response PMID: 18395851
    25. IL12- and CD137L-transfected plasmocytoma cells prevented tumor growth and induced long-lasting immunity PMID: 18610746
    26. Reverse CD137 ligand signaling takes place in hematopoietic progenitor cells, where it induces proliferation and differentiation toward monocytes and macrophages. PMID: 18768847
    27. modification of cancer cells with 4-1BBL gene can produce anti-tumor immune responses. PMID: 18954562
    28. During mild respiratory influenza infection in which virus is rapidly cleared, costimulatory receptor 4-1BB is transiently induced on lung T cells and 4-1BB ligand (4-1BBL) is completely dispensable for the initial CD8 T cell response and mouse survival. PMID: 19124736
    29. 41BBL requires hexamerization for activation PMID: 19596991
    30. CD137L ordinarily regulates the GC B-cell response and thereby acts as a tumor suppressor. PMID: 19608748
    31. These findings demonstrate that reverse signals evoked by CD137L regulate immune functions in macrophages. PMID: 19676073
    32. 4-1BB ligand induces cell division, sustains survival, and enhances effector functions of CD4 and CD8 T cells under comparable conditions of antigenic stimulation. PMID: 11466348

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Tumor necrosis factor family
  • 數(shù)據(jù)庫鏈接：