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  4. Mouse Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit

Mouse Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit

  • 中文名稱(chēng)：
    小鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2/Flk-1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04764m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2/Flk-1)酶聯(lián)免疫試劑盒（CSB-E04764m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的VEGFR-2/Flk-1含量。VEGFR-2（血管內(nèi)皮生長(zhǎng)因子受體-2）是VEGF（血管內(nèi)皮生長(zhǎng)因子）的主要受體，在血管生成和腫瘤生長(zhǎng)中發(fā)揮關(guān)鍵作用。其研究機(jī)制涉及VEGF與VEGFR-2結(jié)合后激活下游信號(hào)通路，如Ras/Raf/MAPK和PI3K/Akt，進(jìn)而影響細(xì)胞增殖、存活和遷移。VEGFR-2抑制劑已成為抗腫瘤治療的重要靶點(diǎn)。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL，靈敏度為1.95 pg/mL。適用于生命科學(xué)研究中血管生成相關(guān)機(jī)制探討、抗血管生成藥物篩選、腫瘤模型或心血管疾病模型中VEGFR - 2表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)等實(shí)驗(yàn)場(chǎng)景，為研究血管生物學(xué)及病理生理過(guò)程提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Kdr ELISA Kit; Flk-1 ELISA Kit; Flk1 ELISA Kit; Vascular endothelial growth factor receptor 2 ELISA Kit; VEGFR-2 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fetal liver kinase 1 ELISA Kit; FLK-1 ELISA Kit; Kinase NYK ELISA Kit; Protein-tyrosine kinase receptor flk-1 ELISA Kit; CD antigen CD309 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGFR-2/Flk-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 86    
    Range % 82-91    
    1:200 Average % 101    
    Range % 98-105    
    1:400 Average % 87    
    Range % 84-91    
    1:800 Average % 100    
    Range % 95-105    
  • 回收率：
    The recovery of mouse VEGFR-2/Flk-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 85-94    
    EDTA plasma (n=4) 107 103-110    
                   
                   
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    500 2.564 2.536 2.550 2.438    
    250 1.513 1.531 1.522 1.410    
    125 0.985 0.961 0.973 0.861    
    62.5 0.551 0.560 0.556 0.444    
    31.2 0.345 0.351 0.348 0.236    
    15.6 0.242 0.251 0.247 0.135    
    7.8 0.174 0.176 0.175 0.063    
    0 0.113 0.110 0.112 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    VEGFR-2/Flk-1是一種受體蛋白，全名為血管內(nèi)皮生長(zhǎng)因子受體-2/胚胎成纖維母細(xì)胞激素樣激酶-1。它是血管內(nèi)皮生長(zhǎng)因子（VEGF）信號(hào)通路中的重要組成部分，參與血管生成、修復(fù)和再生等生理過(guò)程。VEGFR-2/Flk-1在腫瘤血管形成過(guò)程中也起到了重要作用。
  • 功能：
    Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
  • 亞細(xì)胞定位：
    Cell junction. Endoplasmic reticulum. Cell membrane.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome.; [Isoform 2]: Secreted.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
  • 組織特異性：
    Expressed in endothelial cells (at protein level). Detected in embryonic endothelial cells, as well as hematopoietic stem and progenitor cells. Detected in vascular endothelium. Expressed at high levels in adult heart, lung, kidney, brain and skeletal mus
  • 數(shù)據(jù)庫(kù)鏈接：

    STRING: 10090.ENSMUSP00000109144

    UniGene: Mm.285