Mouse Vascular endothelial cell growth factor receptor 3 (VEGFR-3/Flt-4) ELISA kit
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中文名稱(chēng):小鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體-3(VEGFR-3/Flt-4)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E04766m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體-3(VEGFR-3/Flt-4)酶聯(lián)免疫試劑盒(CSB-E04766m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的FLT4含量。FLT4即Fms樣酪氨酸激酶4,是血管內(nèi)皮生長(zhǎng)因子受體家族成員。它在淋巴管生成中發(fā)揮關(guān)鍵作用,與腫瘤轉(zhuǎn)移、淋巴水腫等疾病密切相關(guān)。研究圍繞其信號(hào)通路,探索抑制或激活機(jī)制,有望為相關(guān)疾病治療提供新思路和靶點(diǎn)。試劑盒檢測(cè)范圍為78 pg/ml-5000 pg/ml.,靈敏度為19.5 pg/ml.。適用于腫瘤微環(huán)境研究、淋巴管發(fā)育機(jī)制探索、轉(zhuǎn)基因動(dòng)物模型驗(yàn)證及抗血管生成藥物開(kāi)發(fā)等科研領(lǐng)域,為探究VEGFR - 3在腫瘤轉(zhuǎn)移、炎癥反應(yīng)或遺傳性淋巴水腫等病理過(guò)程中的表達(dá)動(dòng)態(tài)提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:Flt4 ELISA Kit; Flt-4 ELISA Kit; Vegfr3 ELISA Kit; Vascular endothelial growth factor receptor 3 ELISA Kit; VEGFR-3 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fms-like tyrosine kinase 4 ELISA Kit; FLT-4 ELISA Kit; Tyrosine-protein kinase receptor FLT4 ELISA Kit
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縮寫(xiě):
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類(lèi)型:serum, plasma, tissue homogenates
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檢測(cè)范圍:78 pg/ml-5000 pg/ml.
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靈敏度:19.5 pg/ml.
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線(xiàn)性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGFR-3/Flt-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
95
Range %
88-99
1:2
Average %
90
Range %
85-97
1:4
Average %
96
Range %
90-102
1:8
Average %
98
Range %
95-106
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回收率:
The recovery of mouse VEGFR-3/Flt-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
96
92-103
EDTA plasma (n=4)
99
94-105
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標(biāo)準(zhǔn)曲線(xiàn):
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
5000
3.017
2.834
2.925
2.804
2500
2.300
2.207
2.254
2.132
1250
1.508
1.408
1.458
1.336
625
0.843
0.829
0.836
0.714
312
0.524
0.521
0.523
0.401
156
0.351
0.353
0.352
0.230
78
0.227
0.214
0.221
0.099
0
0.119
0.125
0.122
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數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.
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基因功能參考文獻(xiàn):
- VEGFR3 has a role in lymphatic vessel hyperplasia through cell-autonomous and non-cell-autonomous mechanisms PMID: 29615616
- Fluid shear stress regulates vascular remodeling via VEGFR-3 activation, independently of its ligand, VEGF-C, in the uterus during pregnancy. PMID: 28849193
- Genetic depletion experiments revealed that VEGFR2, but not VEGFR3, is indispensable for maintenance of thyroid vascular integrity. Notably, blockade of VEGF-A or VEGFR2 not only abrogated vascular remodeling but also inhibited follicular hypertrophy, which led to the reduction of thyroid weights during goitrogenesis. PMID: 28438786
- Report the generation of a Vegfr3-CreER (T2) knockin mouse by gene targeting in embryonic stem cells. This mouse expresses the tamoxifen-inducible CreER(T2) recombinase under the endogenous transcriptional control of the Vegfr3 gene without altering its physiological expression or regulation in the lymphatic system. PMID: 26993803
- Lymphangiogenic growth in the diaphragm was highly dependent on vascular endothelial growth factor receptor (VEGFR)-3 signaling. During diaphragm development, macrophages appeared first in a linearly arranged pattern, followed by ingrowth of lymphatic vessels along these patterned lines. PMID: 27464987
- CLEC14A acts in vascular homeostasis by fine-tuning VEGFR-2 and VEGFR-3 signaling in endothelial cells PMID: 27991863
- VEGFR3 limits VEGFR2 expression and VEGF/VEGFR2 pathway activity in quiescent and angiogenic blood vascular endothelial cells, thereby preventing excessive vascular permeability. PMID: 28298294
- Blockade of FLT4 suppresses metastasis of melanoma cells by impaired lymphatic vessels. PMID: 27507214
- Vegf-d and its receptor Vegfr-3 were more highly expressed in lungs of hyperoxic, versus normoxic, wild-type mice, indicating that components of the Vegf-d signalling pathway are up-regulated in hyperoxia. PMID: 26924464
- this study uncovers a unique molecular mechanism of lymphangiogenesis in which galectin-8-dependent crosstalk among VEGF-C, podoplanin and integrin pathways plays a key role. PMID: 27066737
- Tnfsf15, a cytokine produced largely by endothelial cells, facilitates lymphangiogenesis by up-regulating Vegfr3 gene expression in LECs, contributing to the maintenance of the homeostasis of the circulatory system. PMID: 26096340
- Results showed that the VEGF-C/VEGFR-3 system underlies the protective effect of ischemic preconditioning against forebrain ischemia in the mouse hippocampus PMID: 25637798
- Experiments in mice and zebrafish demonstrate that changing levels of VEGFR3/Flt4 modulates aortic lumen diameter consistent with flow-dependent remodeling PMID: 25643397
- The VEGF-C/ VEGFR3 pathway is a positive signal that selectively promotes Neural stem cells activation and conversion into progenitor cells in mice. PMID: 25704818
- Lymphangiogenesis is induced by mycobacterial granulomas via vascular endothelial growth factor receptor-3 and supports systemic T-cell responses against mycobacterial antigen. PMID: 25597700
- Vascular endothelial growth factor C/VEGFR-3 signaling modifies HS and CCL21 gradients around lymphatics, regulating lymphocyte migration. PMID: 25606800
- found that VEGFR2 is required independently of VEGFR3 for endothelial DLL4 up-regulation and angiogenic sprouting, and for VEGFR3 functions in angiogenesis PMID: 25561555
- Leukemic environment is highly enriched with lymphangiogenic stimuli, and that VEGFR-3 inhibition restored NK cell killing function with an increased IFN-gamma level. PMID: 25157650
- reveal the evolutionary conservation of the lymphatic-like phenotype of the Schlemm's canal (SC), implicate VEGF-C and VEGFR-3 as critical regulators of SC lymphangiogenesis PMID: 25061878
- the role of short TRF2 as a preferential core promoter regulator. PMID: 25274728
- VEGF-C/VEGFR3 signaling plays an important role in the resolution of skin inflammation PMID: 24252749
- key role in preventing infections in macrophages PMID: 24656836
- VEGFR-3-mediated lymphangiogenesis thus appears to modulate the folliculogenic microenvironment. PMID: 24036251
- The cornea expresses a novel truncated isoform of this molecule, soluble VEGFR-3, which is critical for corneal alymphaticity and increase corneal transplant survival. PMID: 23476047
- Data suggest a regulatory role for VEGFR-3 in megakaryopoiesis. PMID: 22797697
- results establish that VEGFR2 and VEGFR3 are regulated in a highly differential manner by Notch; successful anti-angiogenic targeting of these receptors and their ligands will strongly depend on the status of endothelial Notch signalling PMID: 22426001
- Suggest that miR-1236 may function as a negative regulator of VEGFR-3 signaling during inflammatory lymphangiogenesis. PMID: 22223733
- Macrophage-derived VEGF-C activates VEGFR-3 in tip cells to reinforce Notch signalling, which contributes to the phenotypic conversion of endothelial cells at fusion points of vessel sprouts. PMID: 21909098
- role of NF-kappaB p50 in regulating the expression of VEGFR-3, Prox1 and lymphatic vessel density in mammary tissue, liver, and lung PMID: 21166921
- VEGFR3 activation by its cognate lignads (VEGFR-C/D)is only crucial to lymphangiogenesis but not required for angiogensis and blood vascular development by modulating VEGFR-2 mediated signals. PMID: 20697430
- systemic inhibition of VEGFR-3 increases inflammatory edema formation and inflammatory cell accumulation despite inhibition of lymphangiogenesis PMID: 20837699
- A VEGF-C-mediated mechanism plays an important role in the expansion of MALT lymphoma and the administration of Flt-4 antibodies had a suppressive effect on tumor growth. PMID: 20586849
- Cell adhesion to extracellular matrix induces a downstream signaling using the tyrosine kinase receptor VEGFR-3 as scaffold. PMID: 20431062
- CLP24 is a novel regulator of VEGFR-2 and VEGFR-3 signaling pathways and of normal lymphatic vessel structure PMID: 20439428
- VEGFR-3 participates in immune cell traffic from peripheral tissues to secondary lymphoid organs by regulating allograft lymphatic vessel CCL21 production PMID: 20231530
- A cooperative function of the three Ras proteins in normal development, and a novel aspect of VEGFR3 signalling modulated by Ras in lymphangiogenesis. PMID: 20179099
- interaction between neuropilin-2 and VEGFR3 mediates proper lymphatic vessel sprouting in response to VEGF-C. PMID: 20065093
- Prox1 synergizes with the p50 of NF-kappaB to control VEGFR-3 expression. PMID: 19901262
- Data suggest that VEGFR-3-specific signaling can induce new blood vessels, to which macrophages contribute a major role, and signify its potential as an additional therapeutic target to the existing antiangiogenesis strategies. PMID: 19808642
- result provides new insights into the involvement of vascular endothelial growth factor 3 signals in the maintenance of vascular integrity through modulation of vascular endothelial growth factor 2 signals PMID: 12393458
- In almost all monocytic-derived cells in conjunctiva. Might function as "trap" or functional "decoy" receptor by binding VEGF-C and VEGF-D growth factors to prevent lymphangiogenesis and angiogenesis into the cornea. PMID: 12614002
- Data demonstrate the expression of vascular endothelial growth factor receptor-3 and vascular endothelial growth factor-C on corneal dendritic cells, which implicate a potential relationship between lymphangiogenesis and leukocyte trafficking in the eye. PMID: 12819011
- VEGFR3 signaling is needed for lymph node metastasis in gastric cancer. PMID: 15072591
- hypoxia-driven vascular development requires the activity of VEGF receptor-3 PMID: 15466359
- Ang1 has a role in lymphatic vessel endothelial proliferation, Tie2 expression, and VEGFR-3 upregulation PMID: 15746084
- Blocking anti-VEGFR3 antibody significantly inhibited not only lymph- but also hemangiogenesis in mice with destrin-mutation in normally avascular cornea. PMID: 15855638
- VEGF receptor 3, normally present on lymphatic and proliferating blood vascular endothelium, is strongly constitutively expressed by corneal epithelium and is mechanistically responsible for suppressing inflammatory corneal angiogenesis PMID: 16849433
- cooperative signaling of VEGFR-2 and -3 is necessary for lymphatic migration and proliferation, but VEGFR-3 is redundant with VEGFR-2 for LEC organization into functional capillaries PMID: 17210781
- Spred1 and spred2 are essential for embrhyonic lymphangiogenesis by regulating VEGFR3 signaling. PMID: 17438136
- VEGFR-3 levels were not changed either in healthy or in diabetic muscle after the exercise training. PMID: 17766486
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亞細(xì)胞定位:Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Note=Ligand-mediated autophosphorylation leads to rapid internalization.
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蛋白家族:Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
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組織特異性:Expressed in adult lung and liver, and in fetal liver, brain, intestine and placenta.
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