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  4. Mouse acetyl-CoA ELISA Kit

Mouse acetyl-CoA ELISA Kit

  • 中文名稱：
    小鼠乙酰輔酶A酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12936m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠乙酰輔酶A酶聯(lián)免疫試劑盒（CSB-E12936m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的acetyl-CoA含量。Acetyl - CoA即乙酰輔酶A，是生物體代謝關(guān)鍵物質(zhì)，在糖、脂肪、蛋白質(zhì)代謝中起樞紐作用。研究發(fā)現(xiàn)，它參與三羧酸循環(huán)、脂肪酸合成等多種代謝途徑，其水平變化會(huì)影響細(xì)胞代謝平衡，也與糖尿病、癌癥等疾病的發(fā)生發(fā)展可能相關(guān)。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，適用于研究能量代謝、脂肪酸合成及細(xì)胞調(diào)控等機(jī)制，特別針對(duì)小鼠模型中的乙酰輔酶A代謝通路探索，例如在代謝性疾病、營(yíng)養(yǎng)干預(yù)或藥物作用等科研場(chǎng)景中，可通過(guò)多種生物樣本進(jìn)行定量分析，為體外實(shí)驗(yàn)和離體組織研究提供可靠工具。滿足科研工作者對(duì)乙酰輔酶A相關(guān)分子機(jī)制的高通量研究需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 縮寫：
    acetyl-CoA
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Acetyl-CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 91  
    Range % 87-93  
    1:40 Average % 103  
    Range % 100-105  
    1:80 Average % 93  
    Range % 87-96  
    1:160 Average % 98  
    Range % 93-101  
  • 回收率：
    The recovery of mouse Acetyl-CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 96 92-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    50 2.192 2.114 2.153 1.996  
    25 1.632 1.647 1.640 1.483  
    12.5 1.124 1.110 1.117 0.960  
    6.25 0.713 0.688 0.701 0.544  
    3.12 0.404 0.421 0.413 0.256  
    1.56 0.291 0.278 0.285 0.128  
    0.78 0.220 0.212 0.216 0.059  
    0 0.158 0.156 0.157 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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