Mouse anti-double stranded DNA antibody(IgM)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (14) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠抗雙鏈DNA抗體/天然DNA抗體(IgM)酶聯(lián)免疫試劑盒（CSB-E12911m）為捕獲法ELISA試劑盒，定量檢測血清、血漿樣本中的dsDNA Ab (IgM)含量。dsDNA Ab (IgM) 即抗雙鏈DNA抗體（IgM類）。在系統(tǒng)性紅斑狼瘡等自身免疫病中，機(jī)體免疫系統(tǒng)失調(diào)產(chǎn)生該抗體，它可與體內(nèi)雙鏈DNA結(jié)合形成免疫復(fù)合物，沉積在組織器官引發(fā)炎癥損傷。深入研究其作用機(jī)制，對疾病診斷和治療有重要意義。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產(chǎn)品可廣泛應(yīng)用于自身免疫疾病機(jī)制研究、藥物開發(fā)中抗體水平動態(tài)監(jiān)測、免疫調(diào)控實(shí)驗(yàn)評估等科研場景，為動物模型中的免疫應(yīng)答分析及病理機(jī)制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

dsDNA Ab (IgM)
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Capture ELISA

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-double stranded DNA antibody (IgM) (dsDNA Ab (IgM)) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-105
1:2	Average %	89
	Range %	85-98
1:4	Average %	93
	Range %	85-97
1:8	Average %	95
	Range %	89-110

回收率：

The recovery of mouse anti-double stranded DNA antibody (IgM) (dsDNA Ab (IgM)) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	83-101
EDTA plasma (n=4)	97	87-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.