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  4. Mouse anti-nuclear Antibody (IgG) ELISA Kit

Mouse anti-nuclear Antibody (IgG) ELISA Kit

  • 中文名稱：
    小鼠抗核抗體IgG酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12912m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠抗核抗體IgG酶聯(lián)免疫試劑盒（CSB-E12912m）為捕獲法ELISA試劑盒，定量檢測血清、血漿樣本中的ANA Ab (IgG)含量。ANA Ab (IgG)即抗核抗體IgG型。ANA是一組針對細胞核內(nèi)多種抗原成分的自身抗體，ANA Ab (IgG)在自身免疫性疾病背景下常出現(xiàn)異常。研究機制上，它可與相應(yīng)抗原結(jié)合，形成免疫復(fù)合物，激活補體等，參與免疫損傷過程。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于科研領(lǐng)域中對小鼠自身免疫模型的研究，包括疾病發(fā)生機制探索、藥物干預(yù)效果評估以及免疫調(diào)節(jié)相關(guān)實驗。適用于動物實驗中的系統(tǒng)性紅斑狼瘡模型、類風濕性關(guān)節(jié)炎模型等自身免疫相關(guān)課題的數(shù)據(jù)采集。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    ANA Ab (IgG)
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Capture ELISA
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-nuclear antibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 84  
    Range % 80-92  
    1:200 Average % 97  
    Range % 91-105  
    1:400 Average % 85  
    Range % 82-89  
    1:800 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of mouse anti-nuclear antibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 92 87-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    500 2.751 2.752 2.752 2.559  
    250 2.327 2.377 2.352 2.159  
    125 1.700 1.675 1.688 1.495  
    62.5 0.993 0.968 0.981 0.788  
    31.2 0.637 0.625 0.631 0.438  
    15.6 0.401 0.416 0.409 0.216  
    7.8 0.299 0.289 0.294 0.101  
    0 0.188 0.197 0.193 　  
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse IgG antibody.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled antigen(100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：血漿（抗凝劑） 1:200稀釋

樣品信息：小鼠

稀釋比：其他 1:200

產(chǎn)品評價： 我用CSB-E12912m檢測狼瘡小鼠和正常對照的血漿，這個試劑盒是我們一直使用的，在很多文獻中也被引用，安全可靠，可重復(fù)性高，價格劃算。身邊的同學也都推薦使用華美，華美的試劑盒十分可靠。

By 廖老師

相關(guān)問答

■ 常見問題解答
Q:

Antibodies for which exactly anti-nuclear proteins are analyzed in this kit?

A:
Thanks for your inquiry.
The kit CSB-E12912m is quantitative determination the general quantity of anti-nuclear antibody (IgG).Pls let me know if you have any further questions. Thank you.