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  4. Mouse brain natriuretic peptide,BNP ELISA Kit

Mouse brain natriuretic peptide,BNP ELISA Kit

  • 中文名稱：
    小鼠腦鈉素/腦鈉尿肽(BNP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07971m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠腦鈉素/腦鈉尿肽(BNP)酶聯(lián)免疫試劑盒（CSB-E07971m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的NPPB含量。NPPB即利鈉肽B前體，在心血管等系統(tǒng)中發(fā)揮重要生理作用，可調(diào)節(jié)體液平衡和血壓。研究發(fā)現(xiàn)其與心力衰竭等疾病密切相關(guān)，通過(guò)其相關(guān)信號(hào)通路機(jī)制可深入了解疾病發(fā)生發(fā)展，有望為相關(guān)疾病的診斷、治療提供新靶點(diǎn)和思路。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，適用于心腦血管疾病動(dòng)物模型研究、藥物心臟安全性評(píng)價(jià)、心肌細(xì)胞功能實(shí)驗(yàn)等科研場(chǎng)景，可精準(zhǔn)分析小鼠樣本中BNP的動(dòng)態(tài)變化，為心血管機(jī)制研究及生物標(biāo)志物探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    NppbNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide ELISA kit; BNP)] ELISA kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-98  
    1:2 Average % 96  
    Range % 90-102  
    1:4 Average % 103  
    Range % 100-107  
    1:8 Average % 89  
    Range % 84-95  
  • 回收率：
    The recovery of mouse BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 90-95  
    EDTA plasma (n=4) 103 101-106  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.877 2.984 2.931 2.774  
    200 2.136 2.015 2.076 1.919  
    100 1.322 1.433 1.378 1.221  
    50 0.811 0.862 0.837 0.680  
    25 0.512 0.514 0.513 0.356  
    12.5 0.322 0.302 0.312 0.155  
    6.25 0.240 0.250 0.245 0.088  
    0 0.153 0.160 0.157    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

這個(gè)試劑盒的標(biāo)準(zhǔn)品是小鼠環(huán)化形式的45AA嗎？我們想檢測(cè)這種形式，而不是線性1-32形式或前體形式。

A:
標(biāo)準(zhǔn)為293細(xì)胞來(lái)源的重組蛋白。

靶點(diǎn)詳情

  • 功能：
    Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis.
  • 基因功能參考文獻(xiàn)：
    1. These results suggested that the papillary tip express a substance that can stimulate BNP production and secretion from cardiomyocytes. PMID: 29734386
    2. The present study provides a novel insight into the potential underlying mechanisms of telmisartan-induced inhibition of cardiomyocyte hypertrophy, which involves inhibition of NFAT activation, nuclear translocation and the ANP/BNP cascade. PMID: 28447738
    3. the developmental regulation and stress response of the Nppa-Nppb cluster involve the concerted action of multiple enhancers and epigenetic changes distributed across a structurally rigid regulatory domain. PMID: 27048739
    4. Data suggest that the negative inhibition of P2X3R activity by the BNP/NPR-A pathway results in a decreased P2X3R-mediated excitability of trigeminal neurons in wildetype cultures. In familial hemiplegic migraine type-1 model cultures, however, lack of efficient P2X3Rs downregulation contributes to the neuronal hyperexcitability phenotype. PMID: 27346147
    5. P2X3 receptors on mouse trigeminal ganglion neurons are subjected to contrasting modulation by inhibitory brain natriuretic peptide and facilitatory calcitonin gene-related peptide that both operate via complex intracellular signaling. PMID: 27175010
    6. mouse trigeminal neurons endogenous BNP acts on NPR-A receptors to determine constitutive depression of P2X3 receptor function. Tonic inhibition of P2X3 receptor activity by BNP/NPR-A/PKG pathways occurs via two distinct mechanisms: P2X3 serine phosphorylation and receptor redistribution to non-raft membrane compartments PMID: 26576636
    7. Data (including data from studies in knockout mice) suggest expression of ANP/BNP/GC-A (atrial natriuretic peptide, brain natriuretic peptide, and guanylyl cyclase-A) is important in embryonic neovascularization and organogenesis. PMID: 26517044
    8. Goalpha plays a role in ANF sorting during intracellular vectorial transport and with the presence of a mechanism that preserves the molar relationship between cellular ANF and BNP. PMID: 25917834
    9. The RNA-Seq demonstrates that NPPB which well activates natriuretic peptide receptor 1 (NPR1), is well expressed in mouse dorsal root ganglian. PMID: 25123163
    10. a slow modulatory action by BNP on TRPV1 and P2X3 receptors outlining the role of this peptide as a negative regulator of trigeminal sensory neuron excitability to nociceptive stimuli PMID: 24312267
    11. These data provide detailed novel insight into the complex effects of natriuretic peptides and their receptors on electrical conduction in the heart. PMID: 24344164
    12. Natriuretic peptides buffer renin-dependent hypertension. PMID: 24717731
    13. BNP-NPRA signaling is involved in both itch and pain and does not function upstream of the GRP-GRPR dedicated neuronal pathway. PMID: 24438367
    14. Data indicate that the identified stress-responsive enhancer of Nppa and Nppb, the most common markers of heart failure, is a 650-bp fragment within 50 kb of the Nppa and Nppb loci. PMID: 24391132
    15. these results define the primary pruriceptive neurons, characterize Nppb as an itch-selective neuropeptide, and reveal the next two stages of this dedicated neuronal pathway. PMID: 23704570
    16. Elevated BNP levels, by inducing sympathetic overdrive and altering Ca(2+) handling, promote adverse cardiac remodelling and ventricular arrhythmias PMID: 22617407
    17. BNP controls early load-dependent regulation of SERCA through calcineurin in heart failure. PMID: 20711735
    18. Expression of brain natriuretic peptide and other serum response factor-dependent fetal cardiac genes in response to acute mechanical stress was blunted in mice lacking Mrtf-A. PMID: 20606005
    19. plasma levels of adrenomedullin, atrial natriuretic peptide(ANP)and BNP increased in association with aging; ANP and BNP increased in association with pulse pressure; possible relation between levels and age-related changes in cardiovascular system PMID: 12484513
    20. BNP-mRNA expression in normotensive AT2-deficient mice demonstrate a direct interaction of the renin-angiotensin system and natriuretic peptide that is fibrosis- rather than blood pressure-dependent. PMID: 14599720
    21. activation of cardiac GC-A by locally secreted atrial and brain natriuretic peptides protects the heart from excessive cardiac remodeling by inhibiting the calcineurin-NFAT pathway. PMID: 15939815
    22. ET-1 and Ang II play a key role in the induction of B-type natriuretic peptide in response to load in intact adult murine hearts. PMID: 17188849
    23. BNP is a specific HIF-1alpha target gene PMID: 17822384
    24. mephrin A initially truncated BNP in kidney and the truncated BNP was catabolized by neprilysin PMID: 17823376
    25. The results show that B-type natriuretic peptide decreases intragastric pressure, emptying and absorption by acting via the NPR-A receptor. PMID: 18367637
    26. BNP promotes vessel growth by increasing the number of endothelial progenitors and enhancing their functional properties. PMID: 19275472
    27. endogenous ANP/BNP protects the heart against the death and progression of pathological remodeling in a mouse model of dilated cardiomyopathy and sudden death PMID: 19346456
    28. Targeting of BNP with short interfering RNA (siRNA) resulted in the inhibition of embryonic stem cell proliferation, as indicated by a marked reduction in the cell number and colony size. PMID: 19399180
    29. Data indicate that EET-mediated cardioprotection involves BNP and PI3K signalling events. PMID: 19401302
    30. The natriuretic peptide/guanylyl cyclase--a system functions as a stress-responsive regulator of angiogenesis in mice. PMID: 19487812
    31. Data show that the system was able to measure the induction of hypertrophic markers BNP and ANP in tissue after 2 hrs and 5 hrs of stretch. PMID: 19670825

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  • 亞細(xì)胞定位：
    [Brain natriuretic peptide 45]: Secreted.
  • 蛋白家族：
    Natriuretic peptide family
  • 組織特異性：
    [Brain natriuretic peptide 45]: Expressed abundantly in the ventricle, and in a lesser extent in the atrium (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:18158

    STRING: 10090.ENSMUSP00000099521

    UniGene: Mm.2740