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Mouse calcitonin gene related peptide (CGRP) ELISA kit

  • 中文名稱:
    小鼠降鈣素基因相關(guān)肽(CGRP)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EQ027706MO
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠降鈣素基因相關(guān)肽(CGRP)酶聯(lián)免疫試劑盒(CSB-EQ027706MO)檢測(cè)血清、血漿、組織勻漿樣本中的CGRP含量。CGRP(降鈣素基因相關(guān)肽)是一種神經(jīng)肽,主要在感覺神經(jīng)纖維表達(dá),如C和Aδ感覺纖維,廣泛分布于全身,尤其在血管周圍。CGRP在偏頭痛的發(fā)病機(jī)制中起關(guān)鍵作用,通過促進(jìn)神經(jīng)源性炎癥和顱腦血管擴(kuò)張引發(fā)偏頭痛。近年來,針對(duì)CGRP及其受體的藥物研發(fā)成為偏頭痛治療的熱點(diǎn)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL,支持科研人員探究CGRP在神經(jīng)科學(xué)、藥理學(xué)或疾病模型中的動(dòng)態(tài)變化,例如分析疼痛模型中神經(jīng)遞質(zhì)釋放規(guī)律、評(píng)估藥物對(duì)CGRP通路的調(diào)控作用,或研究心血管疾病與神經(jīng)肽關(guān)聯(lián)機(jī)制。本試劑盒適用于多種生物樣本類型,為神經(jīng)生物學(xué)、免疫學(xué)及代謝性疾病等基礎(chǔ)研究領(lǐng)域提供標(biāo)準(zhǔn)化檢測(cè)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Beta type CGRP、Calcitonin gene-related peptide II、CGRPII、Calcitonin gene-related peptide 2、CALCB
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 86-98  
    1:2 Average % 92  
    Range % 88-97  
    1:4 Average % 90  
    Range % 85-96  
    1:8 Average % 94  
    Range % 88-99  
  • 回收率:
    The recovery of mouse CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-97  
    EDTA plasma (n=4) 96 91-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.342 2.321 2.332 2.178  
    500 1.772 1.760 1.766 1.612  
    250 1.250 1.241 1.246 1.092  
    125 0.799 0.789 0.794 0.640  
    62.5 0.499 0.487 0.493 0.339  
    31.25 0.364 0.341 0.353 0.199  
    15.6 0.253 0.244 0.249 0.095  
    0 0.156 0.152 0.154    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 2 個(gè)評(píng)價(jià)

樣品類型:組織勻漿液 小鼠神經(jīng)節(jié)組織

樣品信息:小鼠

稀釋比:沒有稀釋

產(chǎn)品評(píng)價(jià): ELISA試劑盒在檢測(cè)CGRP時(shí)展現(xiàn)了較高的靈敏度和特異性。在小鼠drg樣本中,重復(fù)性良好。試劑盒的操作簡(jiǎn)便,且廠家提供了詳細(xì)的實(shí)驗(yàn)步驟和在線技術(shù)支持,幫助解決了實(shí)驗(yàn)中的問題。ELISA試劑盒的檢測(cè)范圍(最低和最高濃度)也適應(yīng)實(shí)驗(yàn)的需求。具備高靈敏度,能夠在低濃度下準(zhǔn)確檢測(cè)CGRP。

By 閆老師

樣品類型:組織勻漿液 小鼠肺組織勻漿液

樣品信息:小鼠

稀釋比:1:50

產(chǎn)品評(píng)價(jià): 我使用CSB-EQ027706MO檢測(cè)小鼠肺組織勻漿液樣本,標(biāo)準(zhǔn)曲線的 OD 值在 0.176 - 1.749 之間呈現(xiàn)良好線性關(guān)系,樣本 OD 值大致在 0.2 - 1.7 區(qū)間。試劑盒的重復(fù)性較好,多次平行實(shí)驗(yàn)結(jié)果波動(dòng)比較小。從操作體驗(yàn)來說,試劑預(yù)分裝設(shè)計(jì)十分貼心,減少了繁瑣的準(zhǔn)備步驟,節(jié)省了時(shí)間。而且靈敏度較高,能精準(zhǔn)檢測(cè)到CGRP,為科研數(shù)據(jù)提供了可靠支撐,是一款非常值得信賴的 ELISA 試劑盒。

By 王老師

靶點(diǎn)詳情

  • 功能:
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Calcitonin family
  • 組織特異性:
    Detected in nerve cells of cerebrum, hippocampus and pons/midbrain in newborns, and only in nerve cells of pons/midbrain in adult.
  • 數(shù)據(jù)庫鏈接: