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  4. Mouse carcinoembryonic antign,CEA ELISA Kit

Mouse carcinoembryonic antign,CEA ELISA Kit

  • 中文名稱：
    小鼠癌胚抗原(CEA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13925m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠癌胚抗原(CEA)酶聯(lián)免疫試劑盒（CSB-E13925m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CEACAM5含量。CEACAM5是一種重要靶點(diǎn)。它在多種惡性腫瘤中高表達(dá)，與腫瘤的發(fā)生、發(fā)展、轉(zhuǎn)移等密切相關(guān)。研究機(jī)制主要聚焦于其對(duì)腫瘤細(xì)胞增殖、黏附、侵襲等生物學(xué)行為的影響，科學(xué)家也在探索基于此靶點(diǎn)開發(fā)針對(duì)性的抗癌藥物及療法。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，本產(chǎn)品在小鼠模型中的動(dòng)態(tài)監(jiān)測(cè)對(duì)癌癥機(jī)制研究、治療藥物篩選及療效評(píng)估具有重要意義，適用于腫瘤模型構(gòu)建、基因編輯動(dòng)物驗(yàn)證、腫瘤微環(huán)境分析等科研場(chǎng)景，助力腫瘤生物學(xué)、免疫治療及轉(zhuǎn)化醫(yī)學(xué)等領(lǐng)域的實(shí)驗(yàn)探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Ceacam5 ELISA Kit; Psg30Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; Pregnancy-specific glycoprotein 30 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %85-93
    1:2Average %98
    Range %95-100
    1:4Average %102
    Range %97-107
    1:8Average %94
    Range %91-96
  • 回收率：
    The recovery of mouse CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9791-105
    EDTA plasma (n=4)9390-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.522 2.635 2.579 2.402
    20001.656 1.764 1.710 1.533
    10001.047 1.095 1.071 0.894
    5000.637 0.604 0.621 0.444
    2500.366 0.390 0.378 0.201
    1250.275 0.281 0.278 0.101
    62.50.212 0.230 0.221 0.044
    00.171 0.183 0.177
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.
  • 基因功能參考文獻(xiàn)：
    1. a new mechanistic insight into how the CEA IgV-like N domain participates in cellular events that can have a macroscopic impact in terms of cancer progression and metastasis. PMID: 24388361
  • 亞細(xì)胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor. Apical cell membrane. Cell surface.
  • 蛋白家族：
    Immunoglobulin superfamily, CEA family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:73250

    UniGene: Mm.244138