Mouse circulating immune complex(CIC)ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠循環(huán)免疫復合物(CIC)酶聯(lián)免疫試劑盒（CSB-E16553m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的CIC含量。小鼠循環(huán)免疫復合物（CIC）是體內(nèi)抗原與抗體結合形成的復合物，多數(shù)情況下對機體有益，但在某些情況下，如免疫復合物沉積，可能引起組織損傷和疾病。CIC的研究涉及其形成機制、臨床意義以及與疾病如類風濕關節(jié)炎的關系。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于小鼠自身免疫疾病模型構建、感染性疾病研究中免疫復合物沉積機制探索，或藥物干預后免疫調(diào)節(jié)效應的評估，也可用于免疫學、病理學等基礎研究中對樣本中 CIC 水平進行檢測以探究免疫調(diào)節(jié)機制或評估疾病模型狀態(tài)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

CIC
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	95
	Range %	89-103
1:40	Average %	90
	Range %	81-100
1:80	Average %	91
	Range %	86-99
1:160	Average %	95
	Range %	91-104

回收率：

The recovery of mouse CIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	96	90-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.