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  4. Mouse estrogen,E ELISA Kit

Mouse estrogen,E ELISA Kit

  • 中文名稱：
    小鼠雌激素(E)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07280m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠雌激素(E)酶聯(lián)免疫試劑盒（CSB-E07280m）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的E含量。試劑盒檢測(cè)范圍為25 pg/mL-1000 pg/mL，適用于科研場(chǎng)景如卵巢功能研究、激素替代治療模型驗(yàn)證、環(huán)境污染物對(duì)內(nèi)分泌干擾效應(yīng)評(píng)估等，還可用于生殖生物學(xué)研究、內(nèi)分泌疾病模型構(gòu)建及環(huán)境激素效應(yīng)評(píng)估等。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    E
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    25 pg/mL-1000 pg/mL
  • 靈敏度：
    25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse estrogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-100  
    1:2 Average % 92  
    Range % 86-98  
    1:4 Average % 98  
    Range % 95-105  
    1:8 Average % 90  
    Range % 88-92  
  • 回收率：
    The recovery of mouse estrogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 96 91-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    0 2.411 2.397 2.404    
    25 1.485 1.526 1.506    
    75 0.900 0.858 0.879    
    230 0.564 0.577 0.571    
    500 0.386 0.364 0.375    
    1000 0.182 0.183 0.183    
  • 本試劑盒所含材料：
    • A 96-well Assay plate--The 96-well plate has been pre-coated with goat-anti-rabbit estrogen antibody.
    • Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • HRP-conjugated estrogen(1 x 6 ml) --Bind to the anti-mouse estrogen antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • Anti-mouse estrogen Antibody (1 x 6 ml)--React with both estrogen in the samples/standards and HRP-conjugated estrogen.
    • Wash Buffer (20x concentrate) 1 x 15ml --Wash away unbound or free substances.
    • Substrate A (1 x 7 ml) --Mix with Substrate B and interacts with HRP, eliciting the solution turns blue.
    • Substrate B (1 x 7 ml) --Mix with Substrate A and interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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