Mouse factor B,BF ELISA Kit
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中文名稱:小鼠B因子(BF)酶聯(lián)免疫試劑盒
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貨號:CSB-E04695m
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規(guī)格:96T/48T
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價格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠B因子(BF)酶聯(lián)免疫試劑盒(CSB-E04695m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的BF含量。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL,科研人員可將其應(yīng)用于小鼠感染模型、自身免疫疾病造模、藥物干預(yù)實驗等場景中,定量分析血清或血漿BF水平變化,為探索補體系統(tǒng)在先天免疫中的作用、炎癥反應(yīng)調(diào)控機制或免疫調(diào)節(jié)劑開發(fā)提供可靠數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Cfb ELISA kit; Bf ELISA kit; H2-BfComplement factor B ELISA kit; EC 3.4.21.47 ELISA kit; C3/C5 convertase) [Cleaved into: Complement factor B Ba fragment; Complement factor B Bb fragment] ELISA kit
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縮寫:BF
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma
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檢測范圍:0.312 μg/mL-20 μg/mL
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靈敏度:0.078 μg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 96 Range % 92-104 1:200 Average % 95 Range % 91-99 1:400 Average % 97 Range % 91-105 1:800 Average % 102 Range % 96-108 -
回收率:
The recovery of mouse BF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 86 82-94 EDTA plasma (n=4) 90 85-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. µg/ml OD1 OD2 Average Corrected 20 2.712 2.811 2.762 2.608 10 2.112 2.212 2.162 2.008 5 1.428 1.447 1.438 1.284 2.5 0.857 0.866 0.862 0.708 1.25 0.524 0.512 0.518 0.364 0.625 0.350 0.341 0.346 0.192 0.312 0.268 0.263 0.266 0.112 0 0.154 0.153 0.154 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Elevated activity of plasma dipeptidyl peptidase 4 upon stress can be targeted to reverse tumor immunosuppression P Li, M Liang, J Zhu, J Chen, L Xia, Z Jin,Pharmacological Research,2025
相關(guān)產(chǎn)品
靶點詳情
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功能:Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.
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亞細(xì)胞定位:Secreted.
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蛋白家族:Peptidase S1 family
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數(shù)據(jù)庫鏈接:
STRING: 10090.ENSMUSP00000025229
UniGene: Mm.653
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