Mouse ferritin,FE ELISA Kit

文獻(xiàn)引用 (4) 說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

小鼠鐵蛋白(FE)酶聯(lián)免疫試劑盒（CSB-E08827m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的FE含量。試劑盒檢測范圍為37.5 ng/mL-2400 ng/mL，適用于探究鐵代謝相關(guān)分子機(jī)制、肝臟功能研究、炎癥模型中的鐵穩(wěn)態(tài)評估，或細(xì)胞實驗中鐵蛋白表達(dá)動態(tài)變化的定量監(jiān)測，為體外實驗及動物模型研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

FE
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

37.5 ng/mL-2400 ng/mL
靈敏度：

9.4 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	82-99
1:2	Average %	92
	Range %	88-100
1:4	Average %	93
	Range %	85-100
1:8	Average %	91
	Range %	85-105

回收率：

The recovery of mouse FE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-100
EDTA plasma (n=4)	96	85-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.