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  4. Mouse glucagon-like peptide-1,GLP-1 ELISA Kit

Mouse glucagon-like peptide-1,GLP-1 ELISA Kit

  • 中文名稱：
    小鼠胰高血糖素樣肽1(GLP-1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08118m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠胰高血糖素樣肽1(GLP-1)酶聯(lián)免疫試劑盒（CSB-E08118m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿.樣本中的GLP-1含量。GLP-1（胰高血糖素樣肽-1）是一種由腸道L細胞分泌的激素，能促進胰島β細胞分泌胰島素，抑制胰高血糖素的分泌，并作用于大腦食欲調節(jié)中樞，抑制食欲，從而降低血糖和體重。其研究機制涉及調節(jié)血糖、減重和心血管保護等多重作用。試劑盒檢測范圍為125 pg/ml-8000 pg/ml.，適用于科研領域中對GLP-1表達水平的定量分析，如糖尿病發(fā)病機制研究、代謝調控藥物篩選、腸道激素功能探索等方向，可為體外實驗提供穩(wěn)定可靠的數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    N/A
  • 縮寫：
    GLP-1
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates.
  • 檢測范圍：
    125 pg/ml-8000 pg/ml.
  • 靈敏度：
    31.25 pg/ml
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    104

    Range %

    98-110

    1:2

    Average %

    95

    Range %

    91-105

    1:4

    Average %

    101

    Range %

    94-108

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of mouse GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    89

    84-94

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    8000

    2.397

    2.334

    2.365

    2.173

    4000

    1.701

    1.667

    1.684

    1.492

    2000

    1.162

    1.098

    1.130

    0.938

    1000

    0.788

    0.819

    0.803

    0.611

    500

    0.572

    0.551

    0.562

    0.370

    250

    0.413

    0.423

    0.418

    0.226

    125

    0.302

    0.294

    0.298

    0.106

    0

    0.189

    0.195

    0.192

    　

  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse GLP-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled GLP-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 1 個評價

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1：5

產品評價： 我用 CSB-E08118m檢測自發(fā)性T2DM小鼠db/db小鼠，稀釋比例為1：5，稀釋后濃度較低，檢測出濃度較低。血清較多的話不稀釋最好，實驗結果準確！

By 丁老師