Mouse hypoxia-inducible factor 1α,HIF-1α ELISA Kit
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中文名稱:小鼠低氧誘導(dǎo)因子1α(HIF-1α)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08541m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠低氧誘導(dǎo)因子1α(HIF-1α)酶聯(lián)免疫試劑盒(CSB-E08541m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的HIF1A含量。HIF1A是細(xì)胞對(duì)低氧環(huán)境適應(yīng)的關(guān)鍵調(diào)節(jié)因子。其背景在于在腫瘤、心血管疾病等病理過(guò)程中異常活躍。研究機(jī)制上,它在低氧條件下穩(wěn)定表達(dá),調(diào)控下游基因轉(zhuǎn)錄,參與血管生成、糖代謝等過(guò)程,是當(dāng)下疾病治療靶點(diǎn)研究熱點(diǎn)。試劑盒檢測(cè)范圍為0.625 pg/mL-40 pg/mL,適用于基礎(chǔ)科研中低氧相關(guān)疾病模型的機(jī)制研究,如腫瘤微環(huán)境分析、心血管疾病病理探索或代謝調(diào)控實(shí)驗(yàn),可為體外培養(yǎng)細(xì)胞上清、動(dòng)物血清/血漿樣本中的HIF-1α動(dòng)態(tài)變化提供可靠檢測(cè)方案。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:Hif1aHypoxia-inducible factor 1-alpha ELISA Kit; HIF-1-alpha ELISA Kit; HIF1-alpha ELISA Kit; ARNT-interacting protein ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma
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檢測(cè)范圍:0.625 pg/mL-40 pg/mL
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靈敏度:0.156 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Epigenetics and Nuclear Signaling
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 100 Range % 95-107 1:200 Average % 89 Range % 83-98 1:400 Average % 91 Range % 87-95 1:800 Average % 96 Range % 90-101 -
回收率:
The recovery of mouse HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-95 EDTA plasma (n=4) 94 90-98 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 40 2.253 2.233 2.243 2.186 20 1.346 1.275 1.311 1.254 10 0.792 0.752 0.772 0.715 5 0.468 0.491 0.480 0.423 2.5 0.263 0.264 0.264 0.207 1.25 0.222 0.240 0.231 0.174 0.625 0.118 0.122 0.120 0.063 0 0.055 0.058 0.057 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Taxol alleviates collagen-induced arthritis in mice by inhibiting the formation of microvessels Xu J.et al,Clin Rheumatol. ,2017
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
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基因功能參考文獻(xiàn):
- MiR-497 approximately 195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch1 and HIF1A activity. PMID: 28685750
- HIF-1alpha activation in myeloid cells critically regulates Inflammatory bowel disease progression. PMID: 29967068
- HOXA9 inhibits HIF1A-mediated glycolysis through interacting with CRIP2 to repress cutaneous squamous cell carcinoma development. PMID: 29662084
- Overexpression of HIF1A leads to radioresistance of cervical cancer. PMID: 30355300
- Studies demonstrate a role for HIF-1alpha in regulation of TSP2 expression via primarily a transcriptional mechanism. PMID: 28789925
- HIF1alpha role in the cardiac hypertrophy, apoptosis, and fibrosis.HIF1alpha is partially inhibited in the cardiac fibrosis by p53. PMID: 29729274
- T3 thyroid hormone increased HIF-1alpha protein levels as well as steroid secretion in Leydig cells PMID: 29417848
- Myeloid-specific deletion of Hif1a had no impact on the number of myeloid cells migrating into the eye. PMID: 28112274
- HS-induced PPARalpha-mediated downregulation of PHD1 is a novel pathway for PHD/HIF-1alpha transcriptional regulation. PMID: 29500923
- HIF1alpha promoted macrophage glycolysis metabolism, which induced M1 polarization in mice. PMID: 29386753
- DAPK deficiency leads to excess HIF-1a accumulation, enhanced IL-17 expression and exacerbated experimental autoimmune encephalomyelitis. PMID: 27312851
- activation of HIF signaling in osteoprogenitor cells increases blood levels of the chemokine C-X-C motif ligand 12, which leads to a systemic increase of breast cancer cell proliferation and dissemination through direct activation of the CXCR4 receptor PMID: 29339479
- HIF regulation of HOIL-1L targets the phosphorylated PKC-zeta for degradation and serves as an hypoxia-adaptive mechanism to stabilize the Na,K-ATPase, avoiding significant lung injury. PMID: 29109255
- our study suggests that HIF1A is a common critical downstream mediator for HLH. We propose that HIF1A activation as the consequence of systemic inflammation, cytokine storm, or ligation of TLR may contribute to HLH development. PMID: 28860338
- Decreased HIF-1alpha may contribute to impaired megakaryopoiesis in immune thrombocytopenia. PMID: 28771276
- The results collectively suggest that in the pressure overload heart failure model, HSF1 promoted formation of macrophages by inducing upregulation of HIF-1 expression, through which heart failure was ameliorated. PMID: 29678661
- Hypoxia-inducible factor 1 alpha deficiency is associated with Renal injury in a mouse model of STZ-induced diabetes. PMID: 28774305
- Results showed that Mn-mediated age-related hearing loss involved an increased level of Hif-1alpha protein with less hydroxylation at proline 564 in spiral ganglion neurons. PMID: 27824154
- Aneurysmal-prone factors induced HIF-1alpha can cause overexpression of MMP-2 and MMP-9 and promote aneurysmal progression. PMID: 27363580
- endothelial progenitor cell protected blood-brain barrier integrity after focal ischemia in diabetic mice through upregulation of HIF-1alpha signaling. PMID: 28697748
- HIF-1 dependent profibrogenic mechanisms are operating at the early onset of renal fibrosis but its contribution declines with the progression in mouse unilateral ureteral obstruction model. PMID: 29352658
- HIF1A dependent wound healing angiogenesis in vivo can be controlled by site-specific lentiviral magnetic targeting of SHP2. PMID: 28434868
- Study shows for the first time that mitochondrial defects which are not related to the electron transport chain and Krebs cycle enzymes contribute differently to HIF-1alpha regulation by affecting HIF-1alpha degradation and HIF-1alpha transcription with reactive oxygen species being rather indirectly involved. PMID: 28531964
- Driving IL-10 expression in CD1d(hi)CD5(+) B cells is a key function of the hypoxia-associated transcription factor HIF-1alpha. PMID: 29343683
- Benzopyranyl 1,2,3-triazole compound inhibits HIF-1alpha by accelerating its degradation, and shows much potential as an anti-cancer agent through its ability to suppress tumor growth and angiogenesis. PMID: 27999195
- that HIF-1 plays an important role in hyperglycemia-induced exacerbation of BBB disruption in ischemic stroke PMID: 27425889
- HIF-1alpha/miR-210 pathway is strongly activated in VHL mutated paragangliomas, weakly activated in SDHx mutated PGLs, and not activated in VHLdel- and SDHxwt/VHLwt-PGLs. PMID: 28036268
- Data indicate the important role of hypoxia inducible factor-1 (HIF-1) in the initial progression of immobilization-induced joint contracture, suggesting the possibility of molecular treatment to prevent the progression of joint contracture prior to intervention with physical therapy. PMID: 27352194
- HIF1alpha-regulated profile defines a melanocyte-specific response under hypoxia, and demonstrates the role of HIF1alpha as an invasive cell state gatekeeper in regulating cellular metabolism, chromatin and transcriptional regulation, vascularization, and invasion. PMID: 28168807
- omega-alkynyl arachidonic acid may promote the anti-inflammatory M2 polarization of macrophages in acute myocardial infarction via regulating the cross-talk between PKM2, HIF-1alpha and iNOS. PMID: 28964797
- Herein we report that TGM1 is a HIF-1-regulated gene that is associated with the upregulation of airway epithelial junction proteins, supporting a protective role for HIF-1 in the lung. PMID: 27423780
- the role of hypoxia-inducible factor-1 for myeloid cell function and immune response during colitis, was investigated. PMID: 29261815
- This study demonstrated that Hypoxia-independent mechanisms of HIF-1alpha expression in astrocytes after ischemic preconditioning. PMID: 28063215
- Activation of HIF-1 signaling pathway participates in the Ang II-induced abdominal aortic aneurysm formation in mice PMID: 27497884
- Depletion of KDM3A coincided with the loss of SIAH1 induction and the accumulation of dimethylated H3K9 surrounding the SIAH1 promoter. Interestingly, KDM3A expression was also up-regulated by nutritional stress in a HIF-1alpha dependent manner. PMID: 28843785
- the proangiogenic capacity of implanted periosteal cells is HIF-1alpha regulated and that metabolic adaptations mediate post-implantation cell survival. PMID: 27058876
- Oxidative stress increases in mouse arteriovenous shunts during early maturation, with increased expression of HIF-1alpha and its target genes NOX-2, HO-1, and VEGF-A. PMID: 28163173
- HIF1a transcriptional activity is stimulated by Protein kinase A-dependent phosphorylation PMID: 27245613
- Data indicate that the HIF-1alpha/VEGF-A axis is an essential aspect of tumor immunity. PMID: 29136509
- PROX1 overexpression in DAB2IP-deficient prostate cancer cells could enhance the accumulation of HIF1alpha protein by inhibiting ubiquitin pathway and then consequently induce an epithelial-mesenchymal transition response. PMID: 27476001
- findings suggest that hypoxia, through HIF1A, contributes to the development and progression of pulmonary fibrosis through its regulation of ADORA2B expression on alternatively activated macrophages, cell differentiation, and production of profibrotic mediators PMID: 28701304
- Death receptor5 pathway and mitochondrial pathway, which are likely mediated by HIF-1alpha, contribute to hypoxia-induced spermatocyte apoptosis. PMID: 28444885
- Mechanical low shear stress activates HIF1alpha at atheroprone regions of arteries via nuclear factor-kappaB and Cezanne. HIF1alpha promotes atherosclerosis initiation at these sites by inducing excessive endothelial cell proliferation and inflammation via the induction of glycolysis enzymes. PMID: 28882872
- CRP can upregulate vascular endothelial growth factor-A (VEGF-A) expression by activating hypoxia inducible factor-1alpha (HIF-1alpha) in ADSCs. PMID: 27526687
- induction of HIF-1alpha mediated transcriptional up-regulation of pro-apoptotic/inflammatory signaling contributes to astrocyte cell death during thiamine deficiency. PMID: 29045486
- Smad3 binding to the -335 hypoxia-responsive element of the COL1A2 promoter required HIF-1alpha both in vitro and in kidney lysate from the disease model, suggesting formation of an HIF-1alpha-Smad3 transcriptional complex. Thus, HIF-1alpha-Smad3 has a novel interaction in glomerulosclerosis. PMID: 27503806
- These results suggest that cyclosporine A (CsA) exhibits antifibrotic effects by degrading HIF-1alpha and that the CsA-HIF-1alpha axis provides new insights into therapeutic options for the treatment of pulmonary fibrosis. PMID: 28446589
- HIF-1alpha protein levels in the ipsilateral hemisphere were higher in the WT mice than Par-1 KO mice after glioma cell implantation. PMID: 26463974
- We conclude that HIF-1 is not a major regulator of Vegfa expression during wound healing; rather, it serves to maintain basal levels of expression of Vegfa and its target genes in intact skin, which are required for optimal granulation tissue formation in response to wounding. PMID: 28686658
- hypoxic IL-22 upregulation is dependent on HIF-1alpha PMID: 27534553
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亞細(xì)胞定位:Cytoplasm. Nucleus. Nucleus speckle.
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組織特異性:Ubiquitous.
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