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  4. Mouse intercellular adhesion molecule 1,ICAM-1 ELISA KIT

Mouse intercellular adhesion molecule 1,ICAM-1 ELISA KIT

  • 中文名稱：
    小鼠細(xì)胞間粘附分子1(ICAM-1/CD54)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04575m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠細(xì)胞間粘附分子1(ICAM-1/CD54)酶聯(lián)免疫試劑盒（CSB-E04575m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ICAM1含量。ICAM1即細(xì)胞間黏附分子 -1，在免疫反應(yīng)等生理過(guò)程中發(fā)揮重要作用。其表達(dá)異常與多種疾病相關(guān)，像炎癥、腫瘤等。研究機(jī)制方面，主要聚焦于其參與細(xì)胞黏附、信號(hào)傳導(dǎo)，可作為潛在治療靶點(diǎn)，通過(guò)藥物干預(yù)其活性來(lái)改善病情。試劑盒檢測(cè)范圍為78 ng/mL-5000 ng/mL，靈敏度為19.5 ng/mL。該產(chǎn)品兼容多種生物樣本類型，為研究炎癥機(jī)制、免疫調(diào)控、心血管疾病模型或藥物干預(yù)實(shí)驗(yàn)提供可靠工具，例如可用于評(píng)估小鼠模型中炎癥因子釋放、內(nèi)皮細(xì)胞激活狀態(tài)或免疫細(xì)胞浸潤(rùn)程度。支持科研人員在體外實(shí)驗(yàn)中高效檢測(cè) ICAM - 1 的動(dòng)態(tài)變化，為探索相關(guān)疾病的分子機(jī)制及潛在治療靶點(diǎn)提供技術(shù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Icam1; Icam-1; Intercellular adhesion molecule 1; ICAM-1; MALA-2; MyD10; CD antigen CD54
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    78 ng/mL-5000 ng/mL
  • 靈敏度：
    19.5 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 91-105  
    1:2 Average % 85  
    Range % 80-92  
    1:4 Average % 96  
    Range % 85-105  
    1:8 Average % 93  
    Range % 83-98  
  • 回收率：
    The recovery of mouse ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 82-101  
    EDTA plasma (n=4) 94 86-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    5000 2.555 2.518 2.537 2.390  
    2500 2.047 2.105 2.076 1.929  
    1250 1.531 1.552 1.542 1.395  
    625 0.812 0.803 0.808 0.661  
    312 0.516 0.524 0.520 0.373  
    156 0.312 0.321 0.317 0.170  
    78 0.229 0.225 0.227 0.080  
    0 0.145 0.149 0.147    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    ICAM1是一種細(xì)胞間粘附分子，可以調(diào)節(jié)免疫細(xì)胞在炎癥、感染等情況下的粘附、遷移和激活，具有重要的生物學(xué)功能。最新的研究表明，ICAM1在多種疾病的發(fā)生和發(fā)展中發(fā)揮著重要的作用，如心血管疾病、炎癥性腸病、腫瘤等。近年來(lái)，研究人員也在探索利用ICAM1的功能和機(jī)制開(kāi)發(fā)新型的治療策略。
  • 功能：
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
  • 基因功能參考文獻(xiàn)：
    1. The present study demonstrated that IL-1b may induce ICAM-1 expression, thus enhancing the cohesion between mesenchymal stem cells and endothelial progenitor cells via the p38 MAPK signaling pathway. PMID: 29393395
    2. ICAM-1 overexpressing Mesenchymal Stem Cells could suppress Dendritic Cells maturation according to co-culture methods and suppress the T cell immune response according to the mixed lymphocyte response (MLR) and lymphoblast transformation test (LTT) tests. PMID: 29763906
    3. The results showed that MOVACs transfected with pCMV5-HA-RKIP significantly inhibited TNF-a induced mRNA and protein expression of ICAM-1 and VCAM-1. PMID: 28983072
    4. Endotoxaemia enhanced early venous thrombosis occurs in a TLR-4 and ICAM-1 dependent fashion, and is potentiated by neutropenia. PMID: 27975098
    5. Data show that intercellular adhesion molecule 1 (ICAM-1) and ICAM-2 on B cells are essential for long-lasting cognate T follicular helper (Tfh)-B cell interactions and efficient selection of low-affinity B cell clones for proliferative clonal expansion PMID: 28939548
    6. in T cells, PI3Kdelta attenuates the activation of Rac1, but sustains the activation of Rap1. PMID: 26740009
    7. PTPN22 colocalized with its substrates at the leading edge of cells migrating on surfaces coated with the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1). PMID: 27703032
    8. Blocking CD54 inhibited NK cell-mediated cytotoxicity of the GM-CSF-stimulated Flt3 ligand conventional dendritic cells. NK cell-mediated lysis of GM-CSF-stimulated cDCs was, in part, a result of increased expression of CD54. PMID: 27034402
    9. These results indicate that, whereas T cells use ICAM-1 and -2 for temporary pulmonary entrapment, neutrophils get sequestered and extravasate into inflamed lungs independent of ICAMs. PMID: 26823454
    10. This study indicates that the ANG II effects are, in part, mediated or triggered by Gal-3 together with the related intercellular signaling (ICAM-1 and IL-6), leading to cardiac inflammation and fibrosis. PMID: 27496875
    11. Data suggest CKIP1 (casein kinase 2 interacting protein-1) plays role in resistance to oxidative stress in glomerular mesangial cells in development of diabetic nephropathy; mechanisms appear to include down-regulation of expression of ICAM1 (intercellular adhesion molecule-1) and fibronectin plus activation of Nrf2/ARE (nuclear factor E2-related factor 2/antioxidant response element) antioxidative stress pathway. PMID: 27481061
    12. ICAM1 regulates pathological cardiac remodeling by mediating proinflammatory leukocyte infiltration in the left ventricle and cardiac fibrosis and dysfunction. PMID: 27068635
    13. Its antiatherogenic impact might be associated with a suppressed adhesion to the endothelium due to down-regulation of endothelial ICAM-1 expression. PMID: 26717272
    14. Luteolin may inhibit tumor angiogenesis and tumor cell proliferation by down-regulation of LFA- 3 and PCNA and up-regulation of ICAM-1 in tumor tissue of tumor-bearing mice, thereby achieving its anti-tumor effect. PMID: 26600503
    15. our results further highlight the pleiotropic role of ICAM-1 in T-cell-dependent immune responses, with a major role in Treg cell development and suppressive function. PMID: 26370005
    16. the present results shed light on regulation of expression and function of ICAM-1 on neutrophils and identify it as an additional regulator of neutrophil effector responses in host defense. PMID: 26647392
    17. we identified intracellular adhesion molecule (ICAM)-1, through fibrinogen binding, as a previously unreported mediator of platelet-monocyte interactions PMID: 26215112
    18. miR-222 was transported into recipient endothelial cells by endothelial microparticles and functionally regulated expression of its target protein ICAM-1 in vitro and in vivo. PMID: 26081516
    19. Timely blockade of ICAM-1.LFA-1 interaction prevents disease onset in a mouse model of emphysema. PMID: 26098520
    20. MRTF-A/B depletion results in an increase in the cell surface expression of ICAM-1 and interactions between HAoECs and leukocytes PMID: 26024305
    21. neutrophils are reliant on endothelial activation for adhesion, initially via E-selectin and subsequently, by integrin-mediated binding to ICAM-1 and VCAM-1, combined with CXCL8-dependent chemotaxis. PMID: 26216937
    22. Inhibition of the P2RX7 pathway not only decreased endothelial ICAM-1 expression and leukocyte adhesion but also prevented microglia overactivation, reduced brain injury, and consequently doubled the early survival of septic mice. PMID: 25998681
    23. ICAM-1 in the tumor microenvironment, via restraining efferocytosis of apoptotic tumor cells, can block M2 macrophage polarization through regulation of PI3K/AKT activation, which leads to prevention of tumor metastasis. PMID: 26068788
    24. Activation of Wnt/beta-catenin signaling in AECs attenuated intercellular adhesion molecule 1 (ICAM-1)/vascular cell adhesion molecule 1 (VCAM-1)-mediated adhesion of both macrophages and neutrophils to AECs. PMID: 26351298
    25. miR-141, as a novel repressor of ICAM-1, is involved in the attenuation of MI/R injury via antithetical regulation of ICAM-1 and inflammatory cell infiltration. PMID: 26371161
    26. ICAM-1 and its localization to myofibers in muscles of mdx mice contributes to the dystrophic pathology. PMID: 25728314
    27. ICAM-1 expression can be visualized with in vivo molecular MRI during EAE, and provides an early tracer of disease activity. PMID: 24753465
    28. Anti-OX40L mAb could prolong secondary heart allograft survival based on CD40/CD40L and LFA-1/ICAM-1 blockade. PMID: 25613092
    29. findings reveal a novel function of ICAM-1 in osteogenesis and suggest a new molecular target to improve bone regeneration and repair in inflammatory microenvironments. PMID: 24702024
    30. ICAM-1 can be considered as a rapid-reacting and sustained gravity-regulated molecule in mammalian cells. PMID: 25654110
    31. This report provides evidence for early-stage regulation of ICAM1 by microRNAs, resulting in reduction of genital pathology associated with chlamydial infection. PMID: 25865776
    32. Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules. PMID: 25795756
    33. cell surface levels of endothelial ICAM-1 rather than the inflammatory stimulus or blood-brain barrier integrity influence the pathway of T-cell diapedesis across the blood-brain barrier. PMID: 25545837
    34. Constrained ICAM-1 mobility is important for dendritic cell function, as dendritic cells expressing a high-mobility ICAM-1 mutant lacking the cytoplasmic domain exhibit diminished antigen-dependent conjugate formation and T cell priming. PMID: 25666808
    35. Our results indicate that nicotine can enhance the expression of ICAM-1 and VCAM-1 on mouse cardiac vascular endothelial cell via p38 MAPK signaling pathway, resulting in increased expression of the cellular adhesion molecules ICAM-1 and VCAM-1. PMID: 25803997
    36. ICAM-1 expression by skeletal muscle cells augments myogenesis, and establish a novel mechanism through which the inflammatory response facilitates growth processes in skeletal muscle. PMID: 25281303
    37. RAGE and ICAM-1 are required for the anti-inflammatory functions of protein C PMID: 24876676
    38. ICAM-1 has a role in polymicrobial sepsis; its blockade modulates neutrophil migration and reversing immunosuppression PMID: 24891762
    39. Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment. PMID: 24345805
    40. altered expression of ICAM-1 on thymic epithelium shifts autoimmunity specifically toward peripheral nerves. PMID: 25108020
    41. Hydrogen peroxide exposure upregulates ICAM-1 expression in skeletal muscle. PMID: 24333630
    42. Data suggest that PGE2/Ep4 (prostaglandin E receptor 4; Ptger4) signaling via cAMP/Epac (exchange protein directly activated by cAMP 1; Rapgef3) induces expression of Icam1 (intercellular adhesion molecule 1) in cerebrovascular endothelial cells. PMID: 23317035
    43. These data indicate that L-selectin plays a major role in the development of C protein induced polymyositis, whereas ICAM-1 plays a lesser role, if any. PMID: 24644046
    44. TNF-alpha-induced MMP-9 expression may contribute to the production of sICAM-1 by MC3T3-E1 cells. PMID: 24502696
    45. pretreatment with C66 not only significantly reduced high glucose (HG)-induced over-expressions of VCAM-1, ICAM-1 and MCP-1, but also remarkably inhibited NF-kappaB activation, MAPKs phosphorylation PMID: 24260158
    46. ICAM-1 is imperative for inducing regulatory T cells to produce IL-10 in vivo, which would prevent granuloma formation. PMID: 24103557
    47. Data suggest that ICAM-1 isoforms have unique roles in experimental autoimmune encephalomyelitis (EAE). PMID: 24435747
    48. These studies demonstrate that ICAM-1 immunobiology is highly complex but that individual isoforms, aside from the full-length molecule, make significant contributions to disease development and pathogenesis. [review] PMID: 24795464
    49. Downregulation of ICAM-1 mitigates MC38 colonic adenocarcinoma invasion. PMID: 24360118
    50. beta2 integrin-mediated neutrophil crawling on endothelial ICAM-1 and ICAM-2 is a prerequisite for transcellular neutrophil diapedesis across the inflamed BBB. PMID: 24259506

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, ICAM family
  • 組織特異性：
    Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells.
  • 數(shù)據(jù)庫(kù)鏈接：