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  4. Mouse interleukin 12,IL-12/P40 ELISA KIT

Mouse interleukin 12,IL-12/P40 ELISA KIT

  • 中文名稱：
    小鼠白介素12(IL-12/P40)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07360m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠白介素12(IL-12/P40)酶聯(lián)免疫試劑盒（CSB-E07360m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL12B含量。IL12B是重要靶點。它參與免疫調(diào)節(jié)，所編碼的蛋白是IL-12和IL-23等細胞因子的亞基。研究機制上，圍繞其在自身免疫疾病、腫瘤免疫等領域展開，通過調(diào)控相關細胞因子活性，有望為相關疾病治療提供新策略。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，靈敏度為3.9 pg/mL。適用于基礎科研場景，如小鼠感染模型、炎癥性疾病造模后的組織微環(huán)境分析，或藥物干預實驗中細胞因子網(wǎng)絡調(diào)控研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Il12bInterleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %92
    Range %88-95
    1:10Average %90
    Range %85-92
    1:20Average %89
    Range %84-96
    1:40Average %92
    Range %86-98
  • 回收率：
    The recovery of mouse IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-100
    EDTA plasma (n=4)9387-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.701 2.852 2.777 2.633
    5002.005 2.076 2.041 1.897
    2501.248 1.316 1.282 1.138
    1250.754 0.704 0.729 0.585
    62.50.476 0.450 0.463 0.319
    31.20.311 0.323 0.317 0.173
    15.60.252 0.259 0.256 0.112
    00.148 0.139 0.144 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 基因功能參考文獻：
    1. In macrophages, SCIMP is constitutively associated with the Lyn tyrosine kinase, is required for tyrosine phosphorylation of TLR4, and facilitates TLR-inducible production of the IL-6 and IL-12p40. PMID: 28098138
    2. Results show the overproduction of IL-12/23p40 in dendritic cells, leading to Th1 and Th17 programs in a mouse model of Yersinia enterocolitica-induced reactive arthritis. PMID: 29494692
    3. this study demonstrates that regulation of IL-12p40 by hypoxia-inducible factor-1alpha controls Th1/Th17 responses to prevent mucosal inflammation PMID: 28120851
    4. the nuclear localized CA-VI B selectively promotes IL-12 expression by interaction with protein arginine N-methyltransferase 5 (PRMT5), which reduces symmetric dimethylation of histone H3 arginine 8 modification (H3R8me2s) at Il12 promoters to facilitate chromatin accessibility, selectively enhancing c-Rel binding to the Il12b promoter. PMID: 28739930
    5. High dilutions of antimony modulate cytokines production and macrophage - Leishmania (L.) amazonensis interaction in vitro.( PMID: 28092793
    6. IL-12p40 depletion in mice could promote ectopic bone formation. PMID: 27472064
    7. Data indicate that there was a differential transcription of 381 genes which changed greater than two fold in B1a of p40-/-IL-2Ralpha-/- mice. PMID: 27105495
    8. this study shows that endosomal signalling by Lactobacillus acidophilus that leads to IL-12 production is inhibited by TLR stimulation from the plasma membrane PMID: 27441725
    9. Furthermore, IL-12p40-/- mice showed decreased sensitization to inhaled cockroach antigen after PVM infection when compared to wild-type mice. PMID: 27400340
    10. The study demonstrate a protective role of IL-12Rbeta2 in relapsing-remitting experimental autoimmune encephalomyelitis. PMID: 26857496
    11. Interleukin-12/23p40 plays an essential role in the development of graft-versus-host disease in mice. PMID: 25846718
    12. Data indicate that sera from treated with cholesterol-p40 (Il12b)-siRNA contained significantly reduced concentrations of p40. PMID: 26232431
    13. this study demonstrates viral RNA analog-induced transcription synergy of Il6 and Il12b via IFN regulatory factor (IRF)1 (TLR3-TIR domain-containing adaptor inducing IFN-beta [TRIF] responsive), C/EBPbeta (TLR7-MyD88 responsive), and JunB (all responsive). PMID: 26109639
    14. our results suggest that IL-12p40 acts as a negative regulator of autoimmune cholangitis in IL-2Ralpha-/- mice. Compared to IL-2Ralpha-/- mice, p40-/-IL-2Ralpha-/- mice develop liver fibrosis and reduced colitis. PMID: 24651036
    15. These results imply that a soluble host or Toxoplasma gondii factor is responsible for the bulk of IL-12p40 production in vivo. PMID: 25024368
    16. the assembled p40-CD5L heterodimer does not recapitulate the biological activity of IL-12. PMID: 24821971
    17. The immune-enhancing effect of the Cronobacter sakazakii ES2 phage results in the activation of nuclear factor-kappaB and dendritic cell maturation via the activation of IL-12p40 in the mouse bone marrow. PMID: 24184907
    18. IL-12 does not induce the expression of IL-7 in microglia and macrophages via both IL-12Rbeta2 and IL-12Rbeta1. PMID: 24224652
    19. Data indicate that the GCN2 knockout mice showed reduced inflammatory responses, with decreased IL-6 and IL-12 expression correlating with significant reduction in animal mortality. PMID: 24248597
    20. IL-12p40 contributed most in inducing Th1 and Th17 polarization PMID: 23697689
    21. IL-12p40 deficiency leads to uncontrolled Trypanosoma cruzi dissemination in the spinal cord resulting in neuronal death and motor dysfunction. PMID: 23152844
    22. Klf10 is a transcription factor that regulates the expression of IL-12p40 in bone marrow-derived macrophages. PMID: 23065757
    23. IL-18 and IL-12 exert a synergistic effect on the lungs by inducing MMPs, cathepsins S, and pro-inflammatory cytokines, which may promote pulmonary emphysema and inflammation. PMID: 22908875
    24. Inhibition of IL-12/IL-23 signaling reduces Alzheimer's disease-like pathology and cognitive decline. PMID: 23178247
    25. Novel IL27p28/IL12p40 cytokine suppressed experimental autoimmune uveitis by inhibiting autoreactive Th1/Th17 cells and promoting expansion of regulatory T cells. PMID: 22936807
    26. TLR2 plays a dual role in regulating TLR9-dependent IL-12p40 production in dendritic cells. PMID: 22635254
    27. in mice deficient in IL-12p40, human metapneumovirus infection induced an exacerbated pulmonary inflammatory response and mucus production, altered cytokine response, and decreased lung function PMID: 22606349
    28. reactive oxygen species are important for IL-12 production induced by lactobacillus PMID: 22536449
    29. MKP-1 increases the expression of IL-12 by enhancing the expression of IRF1 PMID: 22464096
    30. extracellular signal regulated kinases modulate IL-12 expression by regulating IkappaB-zeta-dependent binding of NF-kappaB transcription factors to IL-12b gene promoter PMID: 22131231
    31. Francisella tularensis LVS induced innate production of IL-12p(40)2 is required for dendritic cell migration. PMID: 21669537
    32. microbiota negatively regulates host miR-10a expression, which may contribute to the maintenance of intestinal homeostasis by targeting IL-12/IL-23p40 expression. PMID: 22068236
    33. Data show that the p40 vaccine induced specific antibodies to IL-12 and IL-23, which was associated with improvement of intestinal inflammation and fibrosis. PMID: 21424108
    34. dendrittic cells-derived, controls acute infection by Toxoplasma gondii PMID: 21867928
    35. MW and glucocorticoids independently modulate IL-12p40 production through a mechanism involving ERK1/2 hyperactivation and that glucocorticoids can significantly augment MW-induced inhibition of IL-12p40. PMID: 21730055
    36. A distal enhancer in Il12b is the target of transcriptional repression by the STAT3 pathway and requires the basic leucine zipper (B-ZIP) protein NFIL3. PMID: 21566115
    37. Data identified NFIL3 as a regulatory transcription factor in macrophages, controlling IL-12 p40 production. PMID: 21383239
    38. The colitis-prone SJL/J-derived polymorphic variant of IL12b p40 more readily forms p70 heterodimers than does the colitis-resistant C57BL/6 strain-derived p40 variant. PMID: 21321105
    39. PKCdelta is a regulator of IL-12p40/p70 production by dendritic cells and macrophages, driving the healer phenotype during cutaneous leishmaniasis. PMID: 21287553
    40. macrophage-derived Arg I protects hosts against excessive tissue injury caused by worm eggs during acute schistosomiasis by suppressing IL-12/IL-23p40 PMID: 20483789
    41. CD14(neg) B6 mice showed delayed production of IL12p40 in response to necrotic cells PMID: 19840871
    42. These studies demonstrated that IL-12 plays a predominant role in the expansion of effector CD8(+) T-cell immunity against Encephalitozoon cuniculi, which is critical for host survival. PMID: 20308292
    43. there is a critical role for the tyrosine phosphatase activity of SHP-1 for induction of IL-12p40 production in macrophages in response to TLR ligands PMID: 20145200
    44. Results show that IL-12-->IL-27 sequential gene therapy provides a simple but effective approach for eliminating inaccessible residual tumors. PMID: 20139275
    45. IL-12p40 induction in macrophages is directly regulated by the intracellular glutathione-redox status of mouse macrophages in vitro. PMID: 20164428
    46. We suggest that overproduction of NO may account for neuronal death at the spinal cord of T. cruzi-infected IL-12p40KO mice and that IFN-gamma and S100beta may contribute to NOS activation in the absence of IL-12. PMID: 19923851
    47. We found that the production of IL-12 preceded that of other cytokines in response to Influenza A virus lung infection. PMID: 19941386
    48. Langerin-positive cells are not required for initial activation of invariant natural killer T (iNKT) cells, but are required for downstream release of large quantities of IL-12p70 into the serum. PMID: 19923446
    49. protective and agonistic function in mycobacterial infection PMID: 11739515
    50. Heat shock inhibits IL-12 p40 expression through NF-kappa B signalling pathway in murine macrophages PMID: 11792125

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Type I cytokine receptor family, Type 3 subfamily
  • 數(shù)據(jù)庫鏈接：