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  4. Mouse lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

Mouse lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

  • 中文名稱：
    小鼠脂蛋白相關(guān)磷脂酶A2(Lp-PLA2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08321m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠脂蛋白相關(guān)磷脂酶A2(Lp-PLA2)酶聯(lián)免疫試劑盒（CSB-E08321m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PLA2G7含量。PLA2G7即血小板活化因子乙酰水解酶，它能水解血小板活化因子。該靶點(diǎn)與炎癥、心血管疾病等密切相關(guān)。研究機(jī)制方面主要聚焦于其在炎癥信號傳導(dǎo)中的作用，以及對脂質(zhì)代謝的影響，探索其抑制劑有望為相關(guān)疾病治療提供新策略。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，可在科研中用于評估小鼠模型心血管疾病進(jìn)程、炎癥相關(guān)機(jī)制研究或藥物干預(yù)效果分析。為科研人員提供了一種高靈敏度的工具，可廣泛用于基礎(chǔ)醫(yī)學(xué)研究、藥效評價及分子機(jī)制探索等領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Pla2g7 ELISA Kit; Pafah ELISA Kit; Platelet-activating factor acetylhydrolase ELISA Kit; PAF acetylhydrolase ELISA Kit; EC 3.1.1.47 ELISA Kit; 1-alkyl-2-acetylglycerophosphocholine esterase ELISA Kit; 2-acetyl-1-alkylglycerophosphocholine esterase ELISA Kit; LDL-associated phospholipase A2 ELISA Kit; LDL-PLA(2) ELISA Kit; PAF 2-acylhydrolase ELISA Kit
  • 縮寫：
    PLA2G7
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 92  
    Range % 87-102  
    1:200 Average % 95  
    Range % 84-107  
    1:400 Average % 95  
    Range % 83-102  
    1:800 Average % 96  
    Range % 84-105  
  • 回收率：
    The recovery of mouse Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 86-105  
    EDTA plasma (n=4) 96 88-109  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    500 2.715 2.652 2.684 2.565  
    250 1.817 1.913 1.865 1.746  
    125 1.113 1.165 1.139 1.020  
    62.5 0.600 0.627 0.614 0.495  
    31.2 0.380 0.393 0.387 0.268  
    15.6 0.273 0.292 0.283 0.164  
    7.8 0.192 0.202 0.197 0.078  
    0 0.116 0.122 0.119    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent proinflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in lipoprotein particles and uncontrolled proinflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage.
  • 基因功能參考文獻(xiàn)：
    1. Resveratrol downregulates Lp-PLA2 expression by inhibiting oxidative stress via SIRT1 pathway in a mouse model of chronic inflammation. PMID: 28608449
    2. Lp-PLA2 augments the inflammatory response after MI and antagonizes healing by disrupting the balance between inflammation and repair. PMID: 26232205
    3. Plg from mouse plasma contains oxPtdPC adducts that are not affected by the action of Lp-PLA(2), suggesting that linkage to Plg protects oxPtdPCs from metabolism during their transport in the plasma. PMID: 21614211
    4. SAA up-regulates Lp-PLA2 production significantly via a FPRL1/MAPKs./PPAR-gamma signaling pathway. PMID: 23623642
    5. Deletion of Pla2g7 decreases small intestinal polyp and colon tumor incidence in ApcMin/+ mice. PMID: 23361301
    6. Pla2g7 and Tnfrsf21 have been identified as genetic susceptibility to influenza genes in mice. PMID: 22427645
    7. Pla2g7 plays a crucial physiological role in smooth muscle cell differentiation from stem cells, and interactions between Nrf3 and Pla2g7 are essential. PMID: 22247257
    8. The effects of a specific lp-PLA2 inhibitor on atherosclerosis in ApoE-deficient mice and its associated mechanisms, are reported. PMID: 21909350
    9. Studies designed to evaluate the ability of precursor forms of PAF-AH to mature to fully active proteins indicated that the N-terminal end of human and mouse PAF-AH played important and opposite roles in this process. PMID: 20331434
    10. PAF acetylhydrolase activity in the uterus in early pregnancy was not produced locally but probably resulted from the influx of the plasma form of the enzyme PMID: 11833932
    11. deletion of both subunits induces a marked loss of germ cells at an early spermatogenic stage. PMID: 12551946
    12. PAF acetylhydrolases play key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo PMID: 16371369
    13. over-expression of the PAF-AH (I) catalytic subunits induces centrosomal amplification and microtubule disorganization by disturbing intracellular localization of LIS1 PMID: 17903175
    14. inactivation of PAF, produced by TEC, by the overexpression of plasma PAF-AH affects survival, migration, and the angiogenic response of TEC both in vitro and in vivo PMID: 17908960
    15. mouse and human PAF-AHs associate with human HDL particles of different density PMID: 18434304

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  • 亞細(xì)胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 組織特異性：
    Plasma.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:27226

    STRING: 10090.ENSMUSP00000024706

    UniGene: Mm.9277