Mouse low density lipoprotein,LDL ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠低密度脂蛋白(LDL)酶聯(lián)免疫試劑盒（CSB-E13476m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LDL含量。LDL即低密度脂蛋白，是一種運載膽固醇進(jìn)入外周組織細(xì)胞的脂蛋白顆粒。其水平升高易致膽固醇沉積于動脈壁，引發(fā)動脈粥樣硬化等心血管疾病。研究聚焦于降低LDL水平機制，像抑制其合成、促進(jìn)代謝清除等，以防治相關(guān)病癥。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，該產(chǎn)品可廣泛應(yīng)用于小鼠模型的血脂代謝研究、動脈粥樣硬化病理機制探索以及降脂藥物或功能性食品的體外藥效評估本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

LDL
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 ng/mL-500 ng/mL
靈敏度：

1.95 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	87
	Range %	83-90
1:200	Average %	85
	Range %	81-90
1:400	Average %	95
	Range %	92-98
1:800	Average %	100
	Range %	98-104

回收率：

The recovery of mouse LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	98	93-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.