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  4. Mouse low density lipoprotein cholesterol (LDL-C) ELISA kit

Mouse low density lipoprotein cholesterol (LDL-C) ELISA kit

  • 中文名稱：
    小鼠低密度脂蛋白膽固醇(LDL-C)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EQ027860MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠低密度脂蛋白膽固醇(LDL-C)酶聯(lián)免疫試劑盒（CSB-EQ027860MO）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LDL-C含量。LDL-C（低密度脂蛋白膽固醇）是心血管疾病重要風(fēng)險(xiǎn)因素。其水平升高易在血管壁沉積形成斑塊?？蒲芯劢菇档蚅DL-C水平以減少心血管事件。研究機(jī)制包括抑制合成、加速清除等，如開(kāi)發(fā)他汀類、PCSK9抑制劑等藥物來(lái)干預(yù)該靶點(diǎn)。試劑盒檢測(cè)范圍為0.312 μg/mL-20 μg/mL，該產(chǎn)品可廣泛應(yīng)用于小鼠模型中的脂質(zhì)代謝機(jī)制研究、心血管疾病相關(guān)藥物開(kāi)發(fā)、飲食干預(yù)實(shí)驗(yàn)或基因工程動(dòng)物模型的表型分析，為評(píng)估脂質(zhì)代謝異常、動(dòng)脈硬化病理進(jìn)程及潛在治療策略提供可靠的實(shí)驗(yàn)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫：
    LDL-C
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 μg/mL-20 μg/mL
  • 靈敏度：
    0.078 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 86    
    Range % 82-92    
    1:200 Average % 94    
    Range % 91-98    
    1:400 Average % 107    
    Range % 103-114    
    1:800 Average % 89    
    Range % 85-94    
  • 回收率：
    The recovery of mouse LDL-Cspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 88 84-93    
    EDTA plasma (n=4) 91 87-95    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    μg/ml OD1 OD2 Average      
    20 0.105 0.107 0.106      
    10 0.173 0.178 0.176      
    5 0.248 0.255 0.252      
    2.5 0.330 0.345 0.338      
    1.25 0.430 0.443 0.437      
    0.625 0.640 0.682 0.661      
    0.312 0.941 1.011 0.976      
    0 1.752 1.834 1.793      
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days