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  4. Mouse luteinizing hormone (LH) ELISA kit

Mouse luteinizing hormone (LH) ELISA kit

  • 中文名稱：
    小鼠黃體生成素（LH）酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12770m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠黃體生成素（LH）酶聯(lián)免疫試劑盒（CSB-E12770m）為雙抗夾心法ELISA試劑盒，定量檢測樣本中的LH含量。LH即促黃體生成素，是腺垂體分泌的糖蛋白激素。其可刺激睪丸間質(zhì)細胞合成和分泌雄激素，在女性體內(nèi)可促進排卵和黃體生成。研究機制主要圍繞其與下丘腦-垂體-性腺軸的反饋調(diào)節(jié)，對生殖內(nèi)分泌功能舉足輕重。該產(chǎn)品適用于科研領域的小鼠生殖生理研究，例如卵巢功能評估、激素分泌調(diào)控實驗或環(huán)境因素對生殖軸影響的機制探索，為動物模型中的生殖健康研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    LhbLutropin subunit beta ELISA kit; Lutropin beta chain ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit
  • 縮寫：
    LH
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
  • 檢測范圍：
  • 靈敏度：
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse LH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Six vials lyophilized standard (0.5 ml/bottle)--- Dilute a bottle ofthe standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-labeled LH antibody 1 x 6 ml---Act as the detection antibody.
      • HRP-avidin 1 x 11ml --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • Wash Buffer (20 x concentrate) 1 x 15 ml --- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml --- Chromogenic agent A acts as a hydrogen donor.
      • Substrate B 1 x 7 ml --- Chromogenic agent B (including TMB) is used for color developing.
      • Stop Solution 1 x 7 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 數(shù)據(jù)庫鏈接：