Mouse matrix metalloproteinase 3,MMP-3 ELISA kit
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中文名稱:小鼠基質(zhì)金屬蛋白酶3(MMP-3)酶聯(lián)免疫試劑盒
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貨號:CSB-E04678m
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規(guī)格:96T/48T
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價格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠基質(zhì)金屬蛋白酶3(MMP-3)酶聯(lián)免疫試劑盒(CSB-E04678m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿.樣本中的MMP3含量。MMP3,即基質(zhì)金屬蛋白酶3,是一種參與細(xì)胞外基質(zhì)降解的酶,主要在組織重塑、傷口修復(fù)、動脈粥樣硬化進(jìn)展和腫瘤侵襲中發(fā)揮作用。其表達(dá)受多種刺激物調(diào)控,能夠降解多種膠原蛋白和蛋白聚糖,并激活其他MMPs,如MMP-1、MMP-7和MMP-9,在結(jié)締組織重塑中起關(guān)鍵作用。試劑盒檢測范圍為0.312 ng/ml-20 ng/ml,該產(chǎn)品適用于基礎(chǔ)研究中的炎癥模型評估、組織修復(fù)機(jī)制探索或疾病相關(guān)分子通路分析,能夠為小鼠模型中的MMP-3表達(dá)水平檢測提供可靠數(shù)據(jù)支持,滿足細(xì)胞實驗、動物模型及生物樣本分析的科研需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; EMS-2 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; Transin-1 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates.
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檢測范圍:0.312 ng/ml-20 ng/ml
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靈敏度:0.078 ng/ml
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cancer
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
87
Range %
83-95
1:2
Average %
97
Range %
92-102
1:4
Average %
92
Range %
87-96
1:8
Average %
93
Range %
87-98
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回收率:
The recovery of mouse MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
92
88-95
EDTA plasma (n=4)
101
95-108
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
20
2.367
2.264
2.316
2.157
10
1.688
1.655
1.672
1.513
5
0.975
0.999
0.987
0.828
2.5
0.613
0.652
0.633
0.474
1.25
0.346
0.364
0.355
0.196
0.625
0.251
0.266
0.259
0.100
0.312
0.197
0.189
0.193
0.034
0
0.156
0.162
0.159
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Protective effects of Colla Corii Asini Collagen Peptides on D-galactose injection combined with UVB irradiation-induced aging in mice Q Luo, S Zhang, Z Sun, Z Wang, Q Yue, X Sun, L Tian,PloS one,2025
- Protective Effect of Super-Critical Carbon Dioxide Fluid Extract from Flowers and Buds of Chrysanthemum indicum Linnén Against Ultraviolet-Induced Photo-Aging in Mice Zhang X.et al,Rejuvenation research,2015
- Photo-protective activity of pogostone against UV-induced skin premature aging in mice Wang XF.et al,Exp Gerontol.,2016
- Protective Effect of Supercritical-Carbon Dioxide Fluid Extract from Flowers and Buds of Chrysanthemum indicum Linnén against UV-induced Photo-aging in Mice Zhang Xie et al,Rejuvenation Research,2015
產(chǎn)品評價
樣品類型:血清
樣品信息:小鼠
稀釋比:其他 1:10
產(chǎn)品評價: 我用CSB-E04678m檢測小鼠血清基質(zhì)金屬蛋白酶3水平,結(jié)果如圖所示,試劑盒樣本充足,樣本和稀釋液顏色對應(yīng)直觀,使用說明簡潔明了,操作容易上手,數(shù)據(jù)準(zhǔn)確,滿意。
By 劉老師
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
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基因功能參考文獻(xiàn):
- BMAL1 Deficiency Contributes to Mandibular Dysplasia by Upregulating MMP3. PMID: 29276151
- MMP3 contributes to the pathogenesis of ARDS by affecting the pulmonary inflammatory response in female mice in relevant models of lung injury. PMID: 27676418
- endogenously secreted chemerin plays an autocrine/paracrine role in white adipose tissue, identifying chemerin as a therapeutic target to modulate adipose remodelling. PMID: 27461525
- Overexpression of Mmp3 in 3T3-L1 preadipocytes inhibited differentiation. High fat diet-induced obesity downregulates adipocyte MMP3 expression to trigger adipogenesis, and adipocyte TIMP4 may modulate this process to regulate hyperplastic vs. hypertrophic adipose tissue expansion, fat distribution, and metabolic health in a sex- and depot-dependent manner. PMID: 27879248
- Matrix metalloprotease 3 (MMP3), an endogenous neuronal activator of microglia, increased cytokine release from YAC128 microglia compared to wildtype microglia. We found elevated MMP levels in Huntington's Disease (HD) CSF, and MMP levels correlate with disease severity in HD. These data support a novel role for MMPs and microglial activation in HD pathogenesis. PMID: 27033979
- These results indicate that periodic induction, via use of an eye drop, of AAV-mediated secretion of MMP-3 into aqueous humour could have therapeutic potential for those cases of glaucoma that are sub-optimally responsive to conventional pressure-reducing medications. PMID: 28158775
- Data show that loss of loss of matrix metalloproteinase-3 (MMP-3) repressed the upregulation of the chemokines monocyte chemoattractant protein (MCP)-1 and (C-X-C motif) ligand 1 (CXCL1). PMID: 27809288
- Mmp3-knockout mice maintained higher arterial oxygenation compared to wild-type mice in a model of acute lung injury. PMID: 27096327
- NMP4 deficiency suppressed the arthritis-induced increase in bone resorption, expression of RANKL and MMP-3 mRNA. PMID: 26378628
- MMP-3 produced in blood vessel endothelial cells after spinal cord injury serves as an endogenous molecule for microglial activation followed by p38MAPK activation and proNGF production PMID: 26079709
- genetic inactivation of MMP-3 has profound effects on the structural integrity and plasticity response of the visual cortex of adult mice PMID: 24957860
- Study suggests that MMP-3 modulates intracellular MAP kinase signaling pathway and as such, influences a molecular chaperone that regulates cytoskeletal components important for neurite outgrowth, neuronal migration and dendritic arborization PMID: 25652596
- Data indicate that interferon regulatory factor-8 (IRF8) regulates tumor behavior in an matrix metalloproteinase 3 (MMP3)-dependent manner. PMID: 26008967
- Established are novel molecular mechanisms underlying oxidative stress-mediated dopaminergic neuronal death in which MMP3 activation is a key upstream event that leads to Nox1 induction and eventual dopaminergic neuronal death. PMID: 25536219
- These results demonstrated the role of MMP-3 in blood-spinal cord disruption after spinal cord injury for the first time PMID: 25325922
- study demonstrates that MMP-3 leads to caspase-9 activation and suggests that this occurs indirectly via a cytosolic protein, possibly involving Apaf-1 PMID: 25285627
- Poly(P) induces MMP-3-regulated proliferation and differentiation of induced pluripotent stem cells into odontoblast-like cells. PMID: 25662160
- a novel role of MMP3 in nucleus during viral infection PMID: 24416274
- an extracellular regulator of the Wnt signaling pathway and mammary stem cell activi PMID: 23871604
- MMP-3 has a role in nigrostriatal dopaminergic neuronal loss, BBB damage, and neuroinflammation in an MPTP mouse model of Parkinson's disease PMID: 23853428
- Elevated expression of MMP-3 in globoid cell leukodystrophy mediates pathophysiological processes in this disease. PMID: 23404611
- hnRNP-K regulates extracellular matrix, cell motility, and angiogenesis pathways. Involvement of the selected genes (Cck, Mmp-3, Ptgs2, and Ctgf) and pathways was validated by gene-specific expression analysis PMID: 23564449
- These experiments further define the molecular mechanism of estrogen's bone-protective effects by inducing osteoclast apoptosis through upregulation of MMP3 and FasL cleavage. PMID: 22927007
- the hemopexin domainof MMP3 directs epithelial invasion and branching PMID: 23592797
- These results demonstrate a critical role for MMP3 in motor endplate remodeling, and reveal a potential target for therapeutic intervention to prevent motor endplate degradation following nerve injury. PMID: 23281061
- Data indicate significant upregulation of MMP-9 and -3 expressions and activities in stomach with increasing doses and duration of indomethacin. PMID: 22959068
- Ras-GRF1 and -GRF2 may act as adaptors that bind PLCgamma1 and restrict Ca2+ signalling to the vicinity of focal adhesions, indicating a new role for these GRFs that is required for IL-1 induction of the Ras-->ERK pathway and MMP-3 expression PMID: 23145787
- Activation of epithelial-mesenchymal transition by MMP-induced expression of Rac1b gave rise to lung adenocarcinoma. PMID: 22786680
- MMP-3-triggered Rac1 splicing regulation is modulated by hnRNP A1 PMID: 22345078
- Developmental and adult cerebellar defects might contribute to the aberrant motor phenotype observed in MMP-3-deficient mice and suggest an involvement of MMP-3 in mouse cerebellar development. PMID: 22108898
- in vitro and in vivo evidences suggest that MMP-3 plays an inductive role in KIM-1 shedding by PTEC PMID: 22484054
- mRNA levels of MCP-1 and MMP-3 in intervertebral discs significantly diminished and the ability of MCP-1 or MMP-3 expression to respond to TNF-alpha or TWEAK stimulation was significantly reduced as age increased. PMID: 21928379
- Cellular MMP-3 expression was increased by high-dose epinephrine in both skeletal fibroblast and myoblast cell lines. PMID: 21858843
- These observations support a novel role for MMP-3 in the pathogenesis of idiopathic pulmonary fibrosis. PMID: 21871427
- enhanced MMP3 expression by astrocytes in infected MMP9(-/-) mice suggests an active role of resident cells in participating and potentially collaborating with infiltrating cells in regulating blood-brain barrier PMID: 21800363
- MMP-3 mediated activation of MMP-9 is required for efficient neointima formation after carotid ligation in vivo and for vascular smooth muscle cell migration in vitro PMID: 21719762
- MMP-3 is transcriptionally upregulated by Wnt signaling. PMID: 20534975
- Concurrent expression of Mmp-3 with many cardiogenic genes reveals a critical role in Noggin-induced cardiac differentiation of embryonic stem cells. PMID: 19138802
- analysis of allele-specific regulation of matrix metalloproteinase-3 gene by transcription factor NFkappaB PMID: 20360864
- Early induction of MMP-3 was distinct from MMP-9 during endometriosis, which was regulated by c-Fos and TIMP-3. Melatonin suppressed MMP-3 activity and amplified apoptosis while regressing endometriosis through a caspase-3 mediated pathway. PMID: 20609072
- IL-1-induced signaling through focal adhesions leading to MMP3 release and interactions between SHP-2 and PTPalpha are dependent on the integrity of the catalytic domains of PTPalpha. PMID: 20472558
- Matrix metalloproteinase-3 is increased and participates in neuronal apoptotic signaling downstream of caspase-12 during endoplasmic reticulum stress PMID: 20368330
- These results further indicate a role of MMP-3 in the demise of DArgic neurons and suggest MMP-3 as a candidate cellular target for neuroprotective therapy. PMID: 19815046
- EP3 receptor signaling on endothelial cells is essential for the MMP-9 upregulation that enhances tumor metastasis and angiogenesis. PMID: 19799610
- COX-2 activity modulates MMP-9 and-3 activities PMID: 19844242
- The up-regulation of stromelysin-1 (MMP-3) in a spontaneously demyelinating transgenic mouse precedes onset of disease. PMID: 11830584
- Activation of p38 alpha MAPK enhances its expression by mRNA stabilization PMID: 12060661
- MMp3 expression correlates with virulence following neurotropic mouse hepatitis virus infection PMID: 12097550
- MMP-3 impairs adipose tissue development, possibly by affecting food intake and/or adipose tissue-related angiogenesis PMID: 12669125
- MMP-3 induces secondary and tertiary lateral branching of ducts during mid-puberty and early pregnancy. PMID: 12975354
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亞細(xì)胞定位:Secreted, extracellular space, extracellular matrix.
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蛋白家族:Peptidase M10A family
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