Mouse mucin-5 subtype B,MUC5B ELISA Kit

產(chǎn)品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠粘蛋白/粘液素5B(MUC5B)酶聯(lián)免疫試劑盒（CSB-E15067m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MUC5B含量。MUC5B是重要的黏液蛋白靶點(diǎn)。其背景在于它在呼吸道黏液分泌中起關(guān)鍵作用，異常表達(dá)與多種呼吸道疾病如哮喘、慢性阻塞性肺疾病等相關(guān)。研究機(jī)制主要聚焦于調(diào)控其表達(dá)與分泌的信號(hào)通路，以探索干預(yù)策略，改善疾病黏液高分泌狀況。試劑盒檢測(cè)范圍為1.25 ng/mL-80 ng/mL，適用于體外實(shí)驗(yàn)研究，如探究呼吸道疾病模型中黏液分泌調(diào)控機(jī)制、胃腸黏膜屏障功能評(píng)估、腫瘤微環(huán)境中黏蛋白表達(dá)分析等科研場(chǎng)景，為炎癥性疾病、黏膜相關(guān)病理生理學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MUC5B
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

1.25 ng/mL-80 ng/mL
靈敏度：

0.31 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-94
1:2	Average %	96
	Range %	91-102
1:4	Average %	99
	Range %	93-105
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of mouse MUC5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-96
EDTA plasma (n=4)	98	90-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 2 個(gè)評(píng)價(jià)

樣品類型：組織勻漿液肺組織勻漿上清

樣品信息：小鼠

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)：我通過使用CSB-E15067m檢測(cè)了PR8病毒感染的小鼠肺組織勻漿上清，操作簡(jiǎn)單方便易上手，靈敏度非常高，檢測(cè)結(jié)果十分精準(zhǔn)

By 鄧?yán)蠋?/p>

樣品類型：組織勻漿液肺組織勻漿上清

樣品信息：小鼠

稀釋比：沒有稀釋

By 鄧?yán)蠋?/p>