Mouse oxyntomodulin ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠胃泌酸調(diào)節(jié)素酶聯(lián)免疫試劑盒（CSB-E12950m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的oxyntomodulin含量。小鼠胃泌酸調(diào)節(jié)素（OXM）是一種由胃內(nèi)分泌細(xì)胞分泌的肽激素，主要作用是調(diào)節(jié)胃酸分泌和胃腸道功能。其研究機制涉及對胃腸激素、腸道菌群、胃腸道神經(jīng)遞質(zhì)、腦-腸肽、Cajal間質(zhì)細(xì)胞和胃腸電活動的調(diào)節(jié)，以及苦味受體和離子通道的影響。試劑盒檢測范圍為0.312 pg/mL-20 pg/mL，適用于胃腸生理機制、藥物干預(yù)效果評估或相關(guān)疾病模型的體外研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

oxyntomodulin
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.312 pg/mL-20 pg/mL
靈敏度：

0.078 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	102
	Range %	92-108
1:10	Average %	89
	Range %	85-96
1:20	Average %	97
	Range %	91-105
1:40	Average %	92
	Range %	87-96

回收率：

The recovery of mouse oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	91	86-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.