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  4. Mouse pancreatic alpha amylase (AMY2A)ELISA kit

Mouse pancreatic alpha amylase (AMY2A)ELISA kit

  • 中文名稱：
    小鼠胰α淀粉酶(AMY2A)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL001689MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠胰α淀粉酶(AMY2A)酶聯(lián)免疫試劑盒（CSB-EL001689MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的AMY2A含量。AMY2A是一種與淀粉酶相關(guān)的靶點(diǎn)。它在人體消化過(guò)程中發(fā)揮作用，能將淀粉分解為糖類。研究機(jī)制上，聚焦于其基因表達(dá)調(diào)控、酶活性影響因素等，探索其與消化系統(tǒng)疾病的關(guān)聯(lián)，有望為相關(guān)疾病的診斷和治療提供新方向。試劑盒檢測(cè)范圍為9.38 mU/mL-600 mU/mL，適用于體外培養(yǎng)細(xì)胞模型、動(dòng)物實(shí)驗(yàn)樣本的酶活性評(píng)估，以及代謝干預(yù)或病理狀態(tài)下AMY2A動(dòng)態(tài)變化的機(jī)制研究，為探索胰腺功能調(diào)控及消化系統(tǒng)相關(guān)疾病提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Amy2 ELISA Kit; Amy2a ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
  • 縮寫：
    AMY2A
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    9.38 mU/mL-600 mU/mL
  • 靈敏度：
    2.3 mU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-105
    1:2 Average % 92
    Range % 88-96
    1:4 Average % 86
    Range % 80-92
    1:8 Average % 89
    Range % 84-97
  • 回收率：
    The recovery of mouse AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 87-97
    EDTA plasma (n=4) 101 94-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected
    600 2.680 2.714 2.697 2.563
    300 2.164 2.235 2.200 2.066
    150 1.467 1.421 1.444 1.310
    75 0.759 0.811 0.785 0.651
    37.5 0.533 0.564 0.549 0.415
    18.75 0.317 0.326 0.322 0.188
    9.38 0.227 0.241 0.234 0.100
    0 0.128 0.139 0.134 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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