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  4. Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit

Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit

  • 中文名稱：
    小鼠纖溶酶原激活物抑制因子1(PAI-1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07947m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠纖溶酶原激活物抑制因子1(PAI-1)酶聯(lián)免疫試劑盒（CSB-E07947m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SERPINE1含量。SERPINE1是一個備受關(guān)注的靶點。它在多種生理和病理過程中發(fā)揮重要作用，如參與凝血、細(xì)胞遷移等。研究機制上，多聚焦于其在腫瘤進(jìn)展、纖維化疾病等中的作用，通過調(diào)控相關(guān)信號通路來影響疾病發(fā)展，為相關(guān)疾病治療提供潛在方向。試劑盒檢測范圍為0.9 ng/mL-60 ng/mL，適用于心血管疾病模型、血栓形成機制研究以及創(chuàng)傷后組織修復(fù)等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Serpine1 ELISA Kit; Mr1 ELISA Kit; Pai1 ELISA Kit; Planh1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.9 ng/mL-60 ng/mL
  • 靈敏度：
    0.22 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-95  
    1:2 Average % 95  
    Range % 90-100  
    1:4 Average % 94  
    Range % 88-102  
    1:8 Average % 96  
    Range % 91-103  
  • 回收率：
    The recovery of mouse PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-101  
    EDTA plasma (n=4) 95 92-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    60 3.033 3.108 3.071 2.894  
    30 2.476 2.557 2.517 2.340  
    15 1.887 1.785 1.836 1.659  
    7.5 1.117 1.124 1.121 0.944  
    3.75 0.711 0.719 0.715 0.538  
    1.8 0.488 0.468 0.478 0.301  
    0.9 0.329 0.332 0.331 0.154  
    0 0.178 0.176 0.177 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. Involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 數(shù)據(jù)庫鏈接：