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  4. Mouse platelet-derived growth factor CC (PDGF-CC) ELISA Kit

Mouse platelet-derived growth factor CC (PDGF-CC) ELISA Kit

  • 中文名稱：
    小鼠血小板衍生生長因子CC(PDGF-CC)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13404m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠血小板衍生生長因子CC(PDGF-CC)酶聯(lián)免疫試劑盒（CSB-E13404m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PDGFC含量。PDGFC即血小板衍生生長因子C，是PDGF家族成員。它在胚胎發(fā)育、組織修復(fù)等多種生理過程發(fā)揮重要作用。在研究機制上，其通過與受體結(jié)合激活下游信號通路，參與細胞的增殖、遷移等活動，在癌癥等疾病中也有異常表達，為相關(guān)疾病治療帶來新思路。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于心血管疾病模型、腫瘤機制研究、細胞信號通路探索等多種基礎(chǔ)研究場景；其定量分析對研究血管相關(guān)疾病、腫瘤微環(huán)境及再生醫(yī)學(xué)具有重要意義。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Pdgfc ELISA Kit; Scdgf ELISA Kit; Platelet-derived growth factor C ELISA Kit; PDGF-C ELISA Kit; Fallotein ELISA Kit; Spinal cord-derived growth factor ELISA Kit; SCDGF ELISA Kit; VEGF-E) [Cleaved into: Platelet-derived growth factor C ELISA Kit; latent form ELISA Kit; PDGFC latent form); Platelet-derived growth factor C ELISA Kit; receptor-binding form ELISA Kit; PDGFC receptor-binding form)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 101
    Range % 95-105
    1:2 Average % 95
    Range % 89-100
    1:4 Average % 96
    Range % 88-101
    1:8 Average % 94
    Range % 90-99
  • 回收率：
    The recovery of mouse PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 84-93
    EDTA plasma (n=4) 98 90-103
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 2.480 2.411 2.446 2.282
    2000 1.879 1.858 1.869 1.705
    1000 1.151 1.109 1.130 0.966
    500 0.636 0.625 0.631 0.467
    250 0.399 0.389 0.394 0.230
    125 0.287 0.278 0.283 0.119
    62.5 0.215 0.210 0.213 0.049
    0 0.166 0.162 0.164 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function.
  • 基因功能參考文獻：
    1. Results provide both in vitro and in vivo evidence that Mac-1 acting together with LRP1 facilitates PDGF-CC activation by thrombolytic tissue plasminogen activator in the neurovascular unit. The requirement of both Mac-1 and LRP1 for efficient activation of PDGF-CC by tPA provides a novel mechanism that helps limit PDGFRalpha signaling in the neurovascular unit. PMID: 28725968
    2. High glucose-mediated induction of PDGF-C via ChREBP in mesangial cells contributes to the development of glomerular mesangial expansion in diabetes. PMID: 27033449
    3. PDGF-CC neutralization or deficiency was not associated with preservation or accelerated loss of peritubular capillaries, suggesting no significant pro-angiogenic effects of PDGF-CC during renal fibrosis PMID: 26216283
    4. heme oxygenase-1 (HMOX1) activity is critically required for the vascular protective/survival effect of PDGF-CC. PMID: 25267616
    5. Studied survival and antiapoptotic effects of PDGF-C on focal retinal lesions in Ccl2(-/-)/Cx3cr1(-/-) on C57BL/6N [Crb1(rd8)] (DKO rd8) background mice, a model for progressive and focal retinal degeneration. PMID: 24709779
    6. loss of FREM1 function promotes epidermal blistering in Fraser syndrome as a consequence of reduced PDGFC activity, in addition to its stabilising role in the basement membrane PMID: 24046351
    7. Study indicates the role for PDGF-C as a critical regulator of impaired angiogenesis of diabetes. PMID: 23856609
    8. High PDGF-C expression induces progressive fibrosis, chronic inflammation, neoangiogenesis and sinusoidal congestion resulting in hepatocellular carcinoma. PMID: 23929039
    9. PDGF-C promotes tumor growth via a growth promoting effect on hepatic stellate cells that is dependent on the presence of functional PAK-2 PMID: 22362252
    10. Data identified PDGF-CC as an important candidate target gene for antiangiogenic therapy, and PDGF-CC inhibition may be of therapeutic value in treating neovascular diseases. PMID: 20566880
    11. PDGF-CC is critically required for neuronal survival in both brain and retina. Its neuroprotective effect of PDGF-CC is achieved by regulating GSK3beta phosphorylation and expression. PMID: 20231377
    12. During development, PDGF-C expression was widespread and dynamic, and found in somites and their derivatives, in kidney, lung, brain PMID: 11744381
    13. Dominant negative PDGF-C inhibits growth of Ewing family tumor cell lines. PMID: 12032822
    14. Data show that platelet-derived growth factor-C is a potent mitogen for cardiac fibroblasts, and overexpression results in an expansion of the interstitium that induces a secondary sex-dependent hypertrophic response in the cardiomyocytes. PMID: 12875986
    15. role for PDGF-C in bleomycin-induced pulmonary fibrosis. PMID: 12972405
    16. During mouse fetal development, PDGF C was expressed in the mesonephric mesenchyme, prefusion skeletal muscle, cardiac myoblasts, and in visceral and vascular smooth muscle. PMID: 15061151
    17. Pdgfc(-/-) mice die in the perinatal period owing to feeding and respiratory difficulties associated with a complete cleft of the secondary palate. PMID: 15361870
    18. PDGF-C is activated during lung development and is a potent growth factor for mesenchymal cells in vivo. PMID: 16830225
    19. These results suggest that PDGF-C signaling is a new mechanism of cleft palate induced by RA. PMID: 17066417
    20. A method for the generation of conditional knockout alleles for Pdgfc is described. PMID: 17941048
    21. PDGFC induced renal fibrosis involves PDGF-alpha receptor expressing cells and proinflammatory effects in renal interstitial fibrosis PMID: 18184860
    22. some tumors may overcome inhibition of VEGF-mediated angiogenesis through upregulation of PDGF-C PMID: 19111878

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  • 相關(guān)疾病：
    Involved in the development of myocarditis and subsequent fibrosis in the experimental model of coxsackievirus B3-induced chronic myocarditis.
  • 亞細胞定位：
    Cytoplasm, cytosol. Secreted. Nucleus. Cytoplasmic granule. Cell membrane.
  • 蛋白家族：
    PDGF/VEGF growth factor family
  • 組織特異性：
    Mainly expressed in kidney, testis, liver, heart and brain (at protein level). Highly expressed in airway epithelium, interstitial cells and alveolar macrophages in the lung of mice overexpressing IL13. Expressed in the ovaries.
  • 數(shù)據(jù)庫鏈接：