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  4. Mouse melanin-concentrating hormone (PMCH) ELISA kit

Mouse melanin-concentrating hormone (PMCH) ELISA kit

  • 中文名稱：
    小鼠前黑色素聚集激素(PMCH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL018230MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠前黑色素聚集激素(PMCH)酶聯(lián)免疫試劑盒（CSB-EL018230MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PMCH含量。PMCH即前黑素集中激素，參與調節(jié)能量代謝、睡眠覺醒等生理過程。其背景上與肥胖、睡眠障礙等疾病相關。研究機制方面，主要是通過與特定受體結合來傳遞信號，影響下游神經(jīng)元活動，進而調控機體多項功能，是藥物研發(fā)的潛在靶點。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，為研究能量代謝相關疾病機制、神經(jīng)內分泌調控或肥胖模型動物中的分子通路提供可靠工具；科研人員可通過對血清或血漿樣本進行循環(huán)激素水平分析，或結合組織勻漿樣本（如下丘腦等特定器官）探究PMCH的局部表達特征，適用于神經(jīng)生物學、代謝疾病基礎研究及藥物干預效果評估等實驗場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Pmch ELISA Kit; Mch ELISA Kit; Pro-MCH [Cleaved into: Neuropeptide-glycine-glutamic acid ELISA Kit; NGE ELISA Kit; Neuropeptide G-E); Neuropeptide-glutamic acid-isoleucine ELISA Kit; NEI ELISA Kit; Neuropeptide E-I); Melanin-concentrating hormone ELISA Kit; MCH)] ELISA Kit
  • 縮寫：
    PMCH
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PMCH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 86-95    
    1:2 Average % 102    
    Range % 93-106    
    1:4 Average % 94    
    Range % 87-98    
    1:8 Average % 97    
    Range % 93-101    
  • 回收率：
    The recovery of mouse PMCH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 87-95    
    EDTA plasma (n=4) 88 84-93    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    400 2.188 2.265 2.227 2.098    
    200 1.509 1.635 1.572 1.443    
    100 0.970 0.998 0.984 0.855    
    50 0.553 0.563 0.558 0.429    
    25 0.352 0.367 0.360 0.231    
    12.5 0.232 0.244 0.238 0.109    
    6.25 0.182 0.188 0.185 0.056    
    0 0.128 0.129 0.129 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal.
  • 基因功能參考文獻：
    1. our experiments reveal an insulin-dependent activation of MCH neurons in obesity, which contributes via distinct mechanisms to the manifestation of impaired locomotor activity and insulin resistance. PMID: 27926856
    2. Results indicate that olanzapine reduced accumbens nucleus GABAergic transmission and that MCH is necessary for the hypolocomotor effects of olanzapine PMID: 26092201
    3. The present work is the first study to systematically compare the effects of MCH signaling on behavioral and metabolic phenotypes.[meta-analysis] PMID: 24924345
    4. A functional cross-talk between MCH and IL-10 prevents resolution of inflammation in experimental colitis. PMID: 24556508
    5. acute activation of MCH neurons is sufficient, but not necessary, to trigger the transition from NREM to REM sleep and MCH neurons also play a role in the initiation and maintenance of NREM sleep. PMID: 24828644
    6. Sleep induction at a circadian time when all of the arousal neurons are active indicates that MCH stimulation can powerfully counteract the combined wake-promoting signal of the arousal neurons PMID: 23785141
    7. Rat prepro-MCH is processed into three neuropeptides: melanin-concentrating hormone, neuropeptide-glutamic acid-isoleucine (NEI), and Neuropeptide-glycine-glutamic acid (NGE). PMID: 1327720
    8. We conclude that MCH neurons regulate glucose tolerance through signaling molecules other than MCH. PMID: 23365238
    9. MCH was found to promote intestinal tumorigenesis at least in part by inhibiting epithelial cell apoptosis PMID: 22848656
    10. AgRP-expressing neurons play a critical role in mediating the metabolic syndrome and infertility of Lep(ob/ob) mice, whereas MCH-expressing neurons have only a minor role PMID: 22232663
    11. The hypothalamic inputs through MCH play an important role in regulating sensory integration from olfactory pathways. PMID: 21669232
    12. Studies suggest that rat and mouse MCH neurons are differentially involved in anatomical networks that control feeding and the sleep/wake cycle. PMID: 21103352
    13. Data suggest that the increased food intake and increased energy expenditure of Scd1-/- mice are independent of the neuropeptide melanin-concentrating hormone. PMID: 19883709
    14. importance of MCH in integration of energy homeostasis downstream of leptin and, in particular, the role of MCH in regulation of energy expenditure PMID: 12897241
    15. MCH triggers hyperphagia in BRS-3-deficient mice PMID: 14988239
    16. The hypothalamic arousal peptide hypocretin/orexin evoked a direct inward current and increased excitatory synaptic activity and spike frequency in the normally silent MCH neurons. PMID: 15157424
    17. enhanced wheel running activities in mice lacking either MCH or its cognate receptor PMID: 15544841
    18. These studies reinforce the important role of MCH in energy homeostasis and indicate that MCH is a plausible target for antiobesity therapy. PMID: 15731402
    19. MCH ablation improves the long-term outcome for several indicators of the aging process. PMID: 16443777
    20. We propose that alpha-DB plays a role in a structure or regulation mechanism unique to MCH-expressing neurons. PMID: 18314094
    21. MCH has a role in inflammatory processes in the intestine PMID: 18650383
    22. The results of this study support a role for MCH in vigilance state regulation in response to changes in energy homeostasis PMID: 18809470
    23. This study showed increased expression of MCH mRNA with fasting and in mice lacking leptin. It also demonstrated that administration of MCH in the lateral ventricle leads to increased feeding. PMID: 8637571

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    MCH family
  • 組織特異性：
    Predominantly expressed in hypothalamus. Also found in heart, intestine, spleen and testis (spermatogonia, early spermatocytes and Sertoli cells). In brain only mature MCH and NEI peptides are present. In peripheral tissues a large product, encompassing t
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