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  4. Mouse ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) (RNASE2) ELISA kit

Mouse ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) (RNASE2) ELISA kit

  • 中文名稱：
    小鼠核糖核酸酶，核糖核酸酶A家族，（肝臟，嗜酸性粒細(xì)胞源性神經(jīng)毒素）(RNASE2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17924m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠核糖核酸酶，核糖核酸酶A家族，（肝臟，嗜酸性粒細(xì)胞源性神經(jīng)毒素）(RNASE2)酶聯(lián)免疫試劑盒（CSB-E17924m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的RNASE2含量。RNASE2又名核糖核酸酶2，是核糖核酸酶A超家族成員。它在免疫調(diào)節(jié)等方面有重要作用，可參與宿主防御外來(lái)病原體入侵。研究機(jī)制上，其可能通過(guò)降解RNA發(fā)揮生物學(xué)功能，在一些炎癥及免疫相關(guān)疾病研究中受關(guān)注。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL，適用于探究RNASE2在肝臟疾病機(jī)制、嗜酸性粒細(xì)胞相關(guān)炎癥模型或神經(jīng)免疫調(diào)控實(shí)驗(yàn)中的動(dòng)態(tài)表達(dá)，為動(dòng)物模型構(gòu)建、藥物干預(yù)效果評(píng)估及分子通路研究提供可靠工具。滿足基礎(chǔ)科研領(lǐng)域?qū)NASE2蛋白水平的精準(zhǔn)分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Rnase2 ELISA Kit; Ear4 ELISA Kit; Non-secretory ribonuclease ELISA Kit; EC 4.6.1.18 ELISA Kit; Eosinophil cationic-type ribonuclease 4 ELISA Kit; MR-4 ELISA Kit; Ribonuclease 2 ELISA Kit; RNase 2 ELISA Kit
  • 縮寫(xiě)：
    RNASE2
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse RNASE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 85-93
    1:2 Average % 100
    Range % 97-104
    1:4 Average % 86
    Range % 83-89
    1:8 Average % 99
    Range % 95-104
  • 回收率：
    The recovery of mouse RNASE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 88 84-92
    EDTA plasma (n=4) 108 104-112
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 2.441 2.501 2.471 2.358
    100 2.020 1.968 1.994 1.881
    50 1.301 1.324 1.313 1.200
    25 0.725 0.705 0.715 0.602
    12.5 0.407 0.412 0.410 0.297
    6.25 0.304 0.311 0.308 0.195
    3.12 0.195 0.199 0.197 0.084
    0 0.112 0.114 0.113 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.
  • 亞細(xì)胞定位：
    Lysosome. Cytoplasmic granule. Note=Matrix of eosinophil's large specific granule.
  • 蛋白家族：
    Pancreatic ribonuclease family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:53877

    UniGene: Mm.377124