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  4. Mouse soluble myosin heavy chain 1,sMHC-1 ELISA Kit

Mouse soluble myosin heavy chain 1,sMHC-1 ELISA Kit

  • 中文名稱：
    小鼠可溶性肌球蛋白重鏈1(sMHC-1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E11155m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠可溶性肌球蛋白重鏈1(sMHC-1)酶聯(lián)免疫試劑盒（CSB-E11155m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MYH1含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，為研究肌肉相關(guān)疾病模型（如心肌缺血、骨骼肌萎縮等）提供可靠工具；科研人員可通過該產(chǎn)品分析運動生理學(xué)中肌肉適應(yīng)性變化、評估藥物或基因干預(yù)對肌肉功能的影響，或探索肌肉退行性疾病的分子機制；適用于基礎(chǔ)研究、藥效評價及生物標志物篩選等科研場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Myh1 ELISA Kit; Myosin-1 ELISA Kit; Myosin heavy chain 1 ELISA Kit; Myosin heavy chain 2x ELISA Kit; MyHC-2x ELISA Kit; Myosin heavy chain ELISA Kit; skeletal muscle ELISA Kit; adult 1 ELISA Kit
  • 縮寫：
    MYH1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse sMHC-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 85-99    
    1:2 Average % 102    
    Range % 97-106    
    1:4 Average % 85    
    Range % 81-90    
    1:8 Average % 104    
    Range % 100-108    
  • 回收率：
    The recovery of mouse sMHC-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 85-96    
    EDTA plasma (n=4) 87 92-93    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.009 2.061 2.035 1.862    
    500 1.424 1.402 1.413 1.240    
    250 0.980 0.951 0.966 0.793    
    125 0.577 0.589 0.583 0.410    
    62.5 0.380 0.398 0.389 0.216    
    31.2 0.271 0.268 0.270 0.097    
    15.6 0.223 0.215 0.219 0.046    
    0 0.176 0.170 0.173 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Muscle contraction.
  • 基因功能參考文獻：
    1. Data suggest that single muscle immobilization induces a shift of myosin heavy chain (MHC) isoforms composition toward a faster contractile phenotype and decreases the polymorphic profile of single fibres, and that activation of p38 and JNK could be a potential mechanism involved in these contractile phenotype modifications during muscle immobilization. PMID: 27383612
    2. p38alpha/beta MAPKs-mediated coactivator recruitment at a proximal MEF-2 site is important for MyHCIId/x gene regulation in skeletal muscle. PMID: 17210568
    3. The maintenance of the relative expression of slow MHC-1 protein is independent of neuromuscular activity in mice. PMID: 18945940
  • 亞細胞定位：
    Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
  • 數(shù)據(jù)庫鏈接：