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  4. Mouse tartrate-resistant acid phosphatase 5b(TRACP-5b) ELISA Kit

Mouse tartrate-resistant acid phosphatase 5b(TRACP-5b) ELISA Kit

  • 中文名稱：
    小鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08492m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶聯(lián)免疫試劑盒（CSB-E08492m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ACP5含量。ACP5是一種靶點(diǎn)。它與多種生理過程和疾病相關(guān)，在骨代謝等方面發(fā)揮作用。相關(guān)研究主要聚焦于其在疾病發(fā)生發(fā)展中的機(jī)制，如在某些骨病中它的表達(dá)變化及對(duì)細(xì)胞功能的影響，以探索基于ACP5開發(fā)疾病治療的新策略。試劑盒檢測(cè)范圍為0.078 mIU/mL-5 mIU/mL，適用于基礎(chǔ)科研中評(píng)估骨代謝動(dòng)物模型的病理狀態(tài)、篩選調(diào)節(jié)骨吸收的化合物或藥物，亦可用于探究骨骼相關(guān)疾病中破骨細(xì)胞活性變化的分子機(jī)制；為骨生物學(xué)、藥理學(xué)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Acp5 ELISA Kit; T5ap ELISA Kit; Trap ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR-AP ELISA Kit; EC 3.1.3.2 ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.078 mIU/mL-5 mIU/mL
  • 靈敏度：
    0.02 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %100
    Range %96-104
    1:2Average %98
    Range %92-107
    1:4Average %93
    Range %89-97
    1:8Average %95
    Range %90-99
  • 回收率：
    The recovery of mouse TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-102
    EDTA plasma (n=4)9187-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    52.412 2.464 2.438 2.270
    2.52.029 2.007 2.018 1.850
    1.251.655 1.564 1.610 1.442
    0.6251.173 1.129 1.151 0.983
    0.3120.734 0.782 0.758 0.590
    0.1560.491 0.456 0.474 0.306
    0.0780.247 0.227 0.237 0.069
    00.168 0.167 0.168 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse TRACP-5b antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled TRACP-5b antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

相關(guān)問答

■ 常見問題解答
Q:

I’m searching for ELISA kits to detect TRAP in mouse plasma/serum and in culture supernatants of osteoclasts cultured on bovine bone slices.
I saw that Cusabio proposes a kit for the detection of TRAP with the reference CSB-E08492m.
Can you tell me if this kit is specific of TRAP5b produced by the osteoclasts (no detection of TRAP produced by other cells)?
Also, do you know if 100 μL of serum/plasma is necessary for the test or if a dilution of the samples is required?
Finally, as I would like to measure TRAP in culture supernatants, I was wondering if conditioned media (alpha-MEM containing inactivated FBS and penicillin/streptomycin) can be used as samples and if bovine TRAP coming from bone slices or FBS will be detected by the kit.
Thank you in advance for your consideration.

A:
Thanks for your inquiry.
Pls check our answer:
TRACP is a good marker of bone resorption and osteoclast activity.
TRACP especially in the presence of tracp-5a (mainly from macrophages) and tracp-5b (mainly from osteoclasts) existed in the blood.
The determination of tracp-5b can reflect osteoclast activity.
CSB-E08492m is specifically for TRACP-5b, properly used for the mouse matrix samples.
The common samples are serum and plasma. The sample adding volume is 100ul. There is no need to dilute.
As for the TRAP in culture supernatants, pls arrange the preliminary experiment. Pls kindly note detects the mouse TRACP-5b not TRAP.
Any question, pls feel free to contact me.

靶點(diǎn)詳情

  • 功能：
    May play a role in the process of bone resorption. The osteoclastic trap acts on nucleotide tri- and diphosphates with higher affinity, compared with other substrates.
  • 基因功能參考文獻(xiàn)：
    1. TRAP and nidogen-2 co-localized in adipose tissue cells in situ. PMID: 25450682
    2. Uteroferrin is a cytokine secreted by uterine glands in response to progesterone that promotes fetal erythropoiesis at various stages of pregnancy. PMID: 25093463
    3. Supraphysiologic oscillatory fluid shear induces upregulation of osteoclastic activity, measured by tartrate-resistant acid phosphatase activity and formation of mineral resorption pits. PMID: 24275439
    4. Pax6 binds endogenously to the proximal region of the tartrate acid phosphatase (TRAP) gene promoter and suppresses nuclear factor of activated T cells c1 (NFATc1)-induced TRAP gene expression. PMID: 23990468
    5. Serum TRAP levels were lower in mice receiving eggshell calcium citrate malate than those receiving eggshells calcium malate. PMID: 23603074
    6. tartrate resistant acid phosphatase PMID: 23444125
    7. TRAP is not essential for the formation of the epiphyseal vascular network. PMID: 22579636
    8. dephosphorylation of Man6P-containing lysosomal proteins requires the concerted action of Acp2 and Acp5 and is needed for hydrolysis and removal of degradation products PMID: 22158965
    9. Accumulation of vesicles in the cytoplasm of mutant osteoclasts indicates a novel function for tartrate-resistant acid phosphatase in modulating intracellular vesicular transport in osteoclasts PMID: 12243748
    10. USF1 and USF2 play a functional role in RANKL-dependent TRAP expression during osteoclast differentiation. PMID: 12663664
    11. TRACP expression is a target for regulation by the macrophage/osteoclast transcription factor PU.1 and the osteoclast commitment factor MiTF PMID: 14584903
    12. YY1 plays a functional role in RANKL-mediated TRAP gene expression during osteoclast differentiation. PMID: 15563837
    13. distinct tartrate-resistant acid phosphatase promoter regions have distinct roles in gene regulation in transgenic mice PMID: 15590658
    14. MMP-2 and MMP-9 expression by TRAP positive mononuclear and multinucleated cells are involved in articular cartilage destruction in CIA. PMID: 15862156
    15. TRAP release is due to secretion, rather than cell death PMID: 16475168
    16. TRACP is important for polarizing responses in naive T cells to antigen-presented dendritic cells. PMID: 16939395
    17. both synthesis as well as degradation of collagen are increased when TRAP is absent in mice at 8 weeks and 6 months of age, showing that TRAP has an important role in the metabolism of collagen PMID: 17551769
    18. transcriptional activity of the Tracp gene, in pre-osteoclastic cells, is negatively regulated by the binding of PARP-1 protein to a potential consensus sequence located in its promoter region PMID: 18021007
    19. prolonged hypoxia induces the formation of TRAP-positive osteoclast-like cells, suggesting the occurrence of an autocrine mechanism for osteoclastogenesis PMID: 18056037
    20. Over-expression of monomeric tartrate resistant acid phosphatase, but not the dimeric form in adipose tissue leads to early onset spontaneous hyperplastic obesity PMID: 18320034
    21. central role for ACP5 in removal of the Man-6-P recognition marker from lysosomal proteins, which are then dephosphorylated PMID: 18940929
    22. in the absence of RANKL and osteoclast differentiation, STAT6 binds the TRAP promoter after IL-4 treatment and directly enhances TRAP expression PMID: 19801646

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  • 亞細(xì)胞定位：
    Lysosome.
  • 組織特異性：
    Characteristic constituent of osteoclasts.
  • 數(shù)據(jù)庫(kù)鏈接：