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  4. Mouse thyrotropin-releasing hormone,TRH ELISA KIT

Mouse thyrotropin-releasing hormone,TRH ELISA KIT

  • 中文名稱：
    小鼠促甲狀腺素釋放激素(TRH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08147m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠促甲狀腺素釋放激素(TRH)酶聯(lián)免疫試劑盒（CSB-E08147m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清樣本中的TRH含量。TRH即促甲狀腺激素釋放激素，是下丘腦分泌的神經(jīng)激素。其主要作用為刺激腺垂體分泌促甲狀腺激素（TSH），進(jìn)而調(diào)節(jié)甲狀腺功能。研究機(jī)制上，TRH與腺垂體細(xì)胞表面受體結(jié)合，通過特定信號(hào)通路發(fā)揮生理效應(yīng)，在甲狀腺軸調(diào)控等方面意義重大。試劑盒檢測(cè)范圍為2.5 pg/mL-160 pg/mL，適用于探索小鼠甲狀腺功能相關(guān)疾病模型、神經(jīng)內(nèi)分泌調(diào)控機(jī)制、藥物干預(yù)效果評(píng)估等科研領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Trh ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit
  • 縮寫：
    TRH
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum
  • 檢測(cè)范圍：
    2.5 pg/mL-160 pg/mL
  • 靈敏度：
    0.625 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-94
    1:2 Average % 105
    Range % 91-113
    1:4 Average % 94
    Range % 90-98
    1:8 Average % 85
    Range % 80-89
  • 回收率：
    The recovery of mouse TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    160 2.604 2.475 2.540 2.421
    80 1.482 1.408 1.445 1.326
    40 0.917 0.969 0.943 0.824
    20 0.621 0.643 0.632 0.513
    10 0.375 0.386 0.381 0.262
    5 0.296 0.281 0.289 0.170
    2.5 0.168 0.172 0.170 0.051
    0 0.121 0.117 0.119 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問答

■ 常見問題解答
Q:

最近我通過日本NacalaiTecque公司詢問了TRH-ELISA試劑盒的情況。我被告知，您的TRH-ELISA試劑盒只能檢測(cè)到TRH而無法檢測(cè)到前TRH。但這引發(fā)了一個(gè)新問題：您提供大鼠TRH或人類TRH ELISA試劑盒，這些試劑盒如何檢測(cè)不同物種之間的差異？我認(rèn)為最終產(chǎn)品中得到的TRH在不同物種之間并不會(huì)有差異。

A:
我們?cè)诓煌锓N的試劑盒上使用不同的原料。它們有不同的特征和不同的基質(zhì)。對(duì)于不同物種的檢測(cè)結(jié)果的差異，我們應(yīng)以實(shí)際測(cè)量的結(jié)果作為真實(shí)結(jié)果。

靶點(diǎn)詳情

  • 功能：
    Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.
  • 基因功能參考文獻(xiàn)：
    1. co-regulation of the enzyme glutaminyl cyclase and its substrate TRH was reflected by a co-induction of both proteins in reactive astrocytes in proximity of Abeta deposits PMID: 25446989
    2. RXRA and RXRB display specific roles in modulating T3-dependent regulation of thyrotropin releasing hormone. PMID: 23896434
    3. Reversal of hypothyroidism in female patient after treatment with thyrotropin releasing hormone. PMID: 6432893
    4. Data suggest that expression of TRH is up-regulated by CREM (cAMP responsive element modulator protein) in hypothalamus; however, regulation of TRH by triiodothyronine appears independent of CREM or CREB (cAMP responsive element binding protein). PMID: 23000398
    5. Thyrotropin-releasing hormone altered the basal expression of the TSHbeta gene and the responsiveness of the gene to thyroid hormone. PMID: 22792320
    6. Klf4 plays a key role in the maturation of TRH expression in hypothalamic neurons. PMID: 21182892
    7. Data demonstrate that the distribution of hypophysiotropic TRH neurons in mice is vastly different from the pattern in rats, with a dominant occurrence of these neurosecretory cells in the compact part and adjacent regions at the mid-level of the PVN. PMID: 20737594
    8. These demonstrations of PPARgamma effects on hypothalamic Trh transcription in vivo consolidate the role of the TRH neuron as a central integrator of energy homeostasis. PMID: 19900503
    9. study provides functional in vivo data showing that both thyroid hormone receptor beta1 and beta2 isoforms have specific roles in regulating thyrotropin releasing hormone transcription PMID: 14726446
    10. Detailed analysis of TRH-knockout mice might provide insight into physiological significance of endogenous TRH. Present review focuses on phenotypic findings of mice deficient in TRH. PMID: 14751031
    11. Expression of thyrotorpin receptors 1 and 2, key elements in TRH regulation, was modified by thyroid status in the hypothalamus but not in the cortex. PMID: 15225659
    12. TRH is absolutely required for both TSH and TH synthesis but is not necessary for thyrotroph cell development PMID: 16339138
    13. TRH is not essential for pregnancy and lactation, but is required for full function of the lactotrophs, and the PRL mRNA level in the pituitary is regulated by TRH, both directly and indirectly via thyroid hormone PMID: 16484326
    14. Trh is expressed dynamically and specifically in the developing mouse definitive endoderm from E7.0 to E8.5 PMID: 17849455
    15. TRH neurons in the hypothalamic paraventricular nucleus suggest that CB1 endocannabinoids may influence both excitatory and inhibitory inputs of these neurons. PMID: 18818298
    16. provide evidence in mice that TSH participates in this photoperiodic signal transduction PMID: 19015516
    17. Results identify TRH as a modulator of orexin neuron firing in the hypothalamus, and suggest that orexin cell excitation may contribute to the arousal-enhancing actions of TRH. PMID: 19204048
    18. TRH modulates behavioral arousal, in part, through the Hcrt system. PMID: 19321767
    19. Direct receptor-mediated excitation of histaminergic tuberomamillary neurons by TRH demands activation of nonselective cation channels as well as electrogenic sodium/divalent calcium ion exchange. PMID: 19357273
    20. Released TRH is inactivated by an ectopeptidase, pyroglutamyl aminopeptidase II (PPII). Acute administration of alcohol modulates pyroglutamyl amino peptidase II activity and mRNA levels in rat limbic regions. PMID: 15707699
    21. There is considerable evidence linking alcohol consumption, sedation, and thyrotropin-releasing hormone (TRH) in the brain septum. PMID: 15240370

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    TRH family
  • 組織特異性：
    Specifically expressed in hypothalamus and testis.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:22044

    STRING: 10090.ENSMUSP00000006046

    UniGene: Mm.1363