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  4. Mouse tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA kit

Mouse tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA kit

  • 中文名稱：
    小鼠基質(zhì)金屬蛋白酶抑制因子2(TIMP-2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07387m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠基質(zhì)金屬蛋白酶抑制因子2(TIMP-2)酶聯(lián)免疫試劑盒（CSB-E07387m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TIMP2含量。TIMP2 是組織金屬蛋白酶抑制因子 2，在體內(nèi)能抑制金屬蛋白酶活性，維持細胞外基質(zhì)穩(wěn)態(tài)。其研究機制主要聚焦于它在腫瘤發(fā)生、發(fā)展中作用，如抑制腫瘤細胞侵襲、轉(zhuǎn)移，還參與血管生成調(diào)控，有望成為癌癥等疾病治療新靶點。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于體外細胞實驗、動物模型研究及組織樣本分析，為探究TIMP-2在炎癥反應(yīng)、腫瘤微環(huán)境調(diào)控或器官纖維化等領(lǐng)域的分子機制提供可靠工具，適用于分子生物學(xué)、病理機制研究等科研場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Timp2 ELISA Kit; Timp-2 ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit; TIMP-2 ELISA Kit
  • 縮寫：
    TIMP2
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 106  
    Range % 101-113  
    1:40 Average % 84  
    Range % 80-88  
    1:80 Average % 95  
    Range % 90-100  
    1:160 Average % 98  
    Range % 94-105  
  • 回收率：
    The recovery of mouse TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-96  
    EDTA plasma (n=4) 88 84-93  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    8000 2.218 2.351 2.285 2.110  
    4000 1.674 1.607 1.641 1.466  
    2000 1.241 1.194 1.218 1.043  
    1000 0.802 0.853 0.828 0.653  
    500 0.576 0.612 0.594 0.419  
    250 0.349 0.340 0.345 0.170  
    125 0.261 0.267 0.264 0.089  
    0 0.177 0.172 0.175    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Protease inhibitor I35 (TIMP) family
  • 組織特異性：
    Detected in testis, retina, hippocampus and cerebral cortex.
  • 數(shù)據(jù)庫鏈接：