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Mouse γ-Secretase ELISA kit

  • 中文名稱：
    小鼠γ分泌酶酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12142m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠γ分泌酶酶聯(lián)免疫試劑盒（CSB-E12142m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APH1B含量。APH1B 是一種重要靶點。它在γ-分泌酶復(fù)合物中起關(guān)鍵作用。研究發(fā)現(xiàn)其與阿爾茨海默病等神經(jīng)退行性疾病機制相關(guān)，可能參與調(diào)節(jié)淀粉樣蛋白生成。目前圍繞它探索新治療策略，深入了解其作用機制或為相關(guān)疾病防治帶來新思路。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于腦組織樣本分析、疾病模型構(gòu)建、神經(jīng)退行機制探究等科研領(lǐng)域，可支持藥物開發(fā)中酶活性抑制劑的功效評估、轉(zhuǎn)基因動物模型驗證及病理狀態(tài)下γ分泌酶表達(dá)水平動態(tài)監(jiān)測等實驗需求，為探索γ分泌酶在細(xì)胞信號傳導(dǎo)和疾病發(fā)生中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Aph1b; Gamma-secretase subunit APH-1B; APH-1b; Aph-1beta
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse γ-Secretase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %96
    Range %90-102
    1:2Average %102
    Range %97-107
    1:4Average %89
    Range %87-93
    1:8Average %94
    Range %90-98
  • 回收率：
    The recovery of mouse γ-Secretase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10399-106
    EDTA plasma (n=4)9589-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.234 2.356 2.295 2.130
    2501.367 1.378 1.373 1.208
    1250.810 0.824 0.817 0.652
    62.50.492 0.452 0.472 0.307
    31.20.313 0.306 0.310 0.145
    15.60.236 0.250 0.243 0.078
    7.80.205 0.209 0.207 0.042
    00.160 0.169 0.165
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (amyloid-beta precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Probably present in a minority of gamma-secretase complexes compared to APH1A.
  • 基因功能參考文獻(xiàn)：
    1. Loss of Aph1B is associated with memory deficit. PMID: 26942869
    2. Prostaglandin I2 accumulation in neuronal cells activates PKA/CREB and JNK/c-Jun signaling pathways by phosphorylation, which results in APH-1alpha/1beta expression. PMID: 27240539
    3. Abeta oligomers in the cerebrospinal fluid (CSF) further promoted the expression of APH-1alpha/-1beta (by >2.5-fold), which enhances the gamma-cleavage of APP and Abeta deposition during AD progression PMID: 26293690
    4. analysis of hippocampal circuitry via Aph1b-gamma-secretase/neuregulin 1 signalling PMID: 24891237
    5. Upregulation of PS1/gamma-secretase activity may be a risk factor for late onset sporadic Alzheimer's disease. PMID: 22140537
    6. study shows gamma-secretase complexes containing different Aph1 subunits have heterogeneous properties; inactivation of Aph1B gamma-secretase in an Alzheimer disease model led to improvement of disease-relevant features without Notch-related side effects PMID: 19299585

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  • 亞細(xì)胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    APH-1 family
  • 數(shù)據(jù)庫鏈接：