New Delhi metallo-beta-lactamase-1 (NDM-1) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

新德里超級細菌金屬β-內(nèi)酰胺酶-1(NDM-1)酶聯(lián)免疫試劑盒（CSB-E14924）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液樣本中的NDM-1含量。NDM - 1即新德里金屬β - 內(nèi)酰胺酶 - 1，是超級細菌產(chǎn)生的一種耐藥酶。背景上，它的出現(xiàn)使細菌耐藥性問題更嚴峻。研究機制方面，它能水解多種β - 內(nèi)酰胺類抗生素，讓抗生素失去抗菌活性，增加了治療細菌感染的難度，是當前研究熱點。試劑盒檢測范圍為6.25 ng/mL- 400 ng/mL，適用于科研領域對細菌耐藥機制研究、抗生素治療效果評估以及耐藥菌株的流行病學監(jiān)測，為實驗室開展NDM - 1相關分子特性分析、耐藥基因傳播規(guī)律研究提供可靠工具。本試劑盒檢測數(shù)據(jù)可支持微生物學、藥理學及感染性疾病領域的學術研究，助力新型抗菌藥物開發(fā)及耐藥性防控策略制定。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

NDM-1
樣本類型：

serum, plasma, urine samples infected by New Delhi super bacteria
檢測范圍：

6.25 ng/mL- 400 ng/mL
靈敏度：

3.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of NDM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-103
1:2	Average %	92
	Range %	82-99
1:4	Average %	93
	Range %	87-97
1:8	Average %	95
	Range %	89-103

回收率：

The recovery of NDM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	96	87-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.