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  4. Pig Alpha-fetoprotein(AFP) ELISA kit

Pig Alpha-fetoprotein(AFP) ELISA kit

  • 中文名稱：
    豬甲胎蛋白(AFP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL001421PI
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬甲胎蛋白(AFP)酶聯(lián)免疫試劑盒（CSB-EL001421PI）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的AFP含量。AFP即甲胎蛋白，是一種糖蛋白。它在胎兒早期由肝臟和卵黃囊合成。在肝癌等多種惡性腫瘤中會(huì)異常升高，臨床上常用作腫瘤標(biāo)志物輔助診斷。研究機(jī)制集中于其表達(dá)調(diào)控及與腫瘤細(xì)胞增殖、轉(zhuǎn)移等過程的關(guān)聯(lián)，以探尋新的治療靶點(diǎn)。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL，適用于豬模型研究中肝臟疾病機(jī)制探索、腫瘤發(fā)生發(fā)展監(jiān)測(cè)、生殖生理學(xué)研究等科研領(lǐng)域，滿足體外實(shí)驗(yàn)中對(duì)AFP蛋白表達(dá)水平動(dòng)態(tài)變化的精準(zhǔn)追蹤需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    AFPAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 檢測(cè)范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %88-102
    1:2Average %92
    Range %87-96
    1:4Average %88
    Range %82-94
    1:8Average %97
    Range %92-102
  • 回收率：
    The recovery of pig AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8480-88
    EDTA plasma (n=4)9186-95
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.416 2.352 2.384 2.289
    1001.683 1.661 1.672 1.577
    500.945 0.904 0.925 0.830
    250.492 0.459 0.476 0.381
    12.50.302 0.287 0.295 0.200
    6.250.214 0.209 0.212 0.117
    3.120.151 0.145 0.148 0.053
    00.098 0.092 0.095 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 組織特異性：
    Plasma. Synthesized by the fetal liver and yolk sac.
  • 數(shù)據(jù)庫鏈接：