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  4. Pig Corticoliberin(CRH) ELISA kit

Pig Corticoliberin(CRH) ELISA kit

  • 中文名稱：
    豬促腎上腺皮質素釋放素(CRH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL005963PI
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬促腎上腺皮質素釋放素(CRH)酶聯(lián)免疫試劑盒（CSB-EL005963PI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CRH含量。CRH（促腎上腺皮質激素釋放激素）是一種重要的神經(jīng)調節(jié)物質，主要作用是促進腺垂體合成與釋放促腎上腺皮質激素（ACTH）。近年來，研究發(fā)現(xiàn)CRH在慢性疼痛誘發(fā)的焦慮和抑郁中發(fā)揮關鍵作用，其調控痛相關抑郁的神經(jīng)環(huán)路機制已得到揭示。試劑盒檢測范圍為100 pg/mL-1600 pg/mL，適用于基礎科研領域，例如探究豬在環(huán)境壓力、代謝異常或特定病理狀態(tài)下CRH的表達規(guī)律，亦可應用于神經(jīng)生物學、動物生理學及藥理學研究中激素調控網(wǎng)絡的解析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CRHCorticoliberin ELISA kit; Corticotropin-releasing factor ELISA kit; CRF ELISA kit; Corticotropin-releasing hormone ELISA kit
  • 縮寫：
    CRH
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    100 pg/mL-1600 pg/mL
  • 靈敏度：
    100 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig CRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-95  
    1:2 Average % 95  
    Range % 92-101  
    1:4 Average % 82  
    Range % 80-83  
    1:8 Average % 88  
    Range % 85-92  
  • 回收率：
    The recovery of pig CRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 87-97  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1600 0.196 0.184 0.190    
    800 0.322 0.343 0.333    
    400 0.516 0.510 0.513    
    200 1.037 1.100 1.069    
    100 2.158 2.323 2.241    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile.
  • 基因功能參考文獻：
    1. Evidence is provided for porcine corticotropin releasing hormone (CRH) as a quantitative trait locus (QTL) affecting growth and body composition. (corticotropin releasing hormone; CRH; CRF) PMID: 16483545
    2. CRH inhibits local progesterone production from luteal cells in swine corpus lutem. PMID: 20431270
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Sauvagine/corticotropin-releasing factor/urotensin I family
  • 組織特異性：
    Produced by the hypothalamus.
  • 數(shù)據(jù)庫鏈接：