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  4. Pig Ferritin heavy chain(FTH1) ELISA kit

Pig Ferritin heavy chain(FTH1) ELISA kit

  • 中文名稱：
    豬鐵蛋白重鏈(FTH1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL009030PI
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬鐵蛋白重鏈(FTH1)酶聯(lián)免疫試劑盒（CSB-EL009030PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FTH1含量。FTH1即鐵蛋白重鏈1，作為重要的鐵代謝相關(guān)蛋白，在維持細(xì)胞內(nèi)鐵穩(wěn)態(tài)中發(fā)揮關(guān)鍵作用。其背景與鐵離子儲存、運輸密切相關(guān)。研究機制上，它涉及調(diào)控鐵離子釋放，還和氧化應(yīng)激、腫瘤發(fā)生發(fā)展等生理病理過程存在關(guān)聯(lián)，是潛在的治療靶點。試劑盒檢測范圍為4.7 ng/ml- 300 ng/ml，適用于動物營養(yǎng)學(xué)研究中鐵代謝動態(tài)監(jiān)測、豬模型鐵相關(guān)疾病機制探索（如缺鐵性貧血或鐵過載研究），以及畜牧養(yǎng)殖中飼料鐵強化效果評估等科研領(lǐng)域，為體外樣本的FTH1定量分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FTH1 ELISA Kit; FTHFerritin heavy chain ELISA Kit; Ferritin H subunit ELISA Kit; EC 1.16.3.1) [Cleaved into: Ferritin heavy chain ELISA Kit; N-terminally processed] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    4.7 ng/ml- 300 ng/ml
  • 靈敏度：
    1.175 ng/ml
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of pig FTH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    92

    Range %

    85-99

    1:2

    Average %

    89

    Range %

    84-95

    1:4

    Average %

    93

    Range %

    89-98

    1:8

    Average %

    99

    Range %

    95-105

  • 回收率：

    The recovery of pig FTH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    88-102

    EDTA plasma (n=4)

    99

    94-103

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    300

    2.734

    2.634

    2.684

    2.525

    150

    2.183

    2.267

    2.225

    2.066

    75

    1.678

    1.728

    1.703

    1.544

    37.5

    1.122

    1.129

    1.126

    0.967

    18.75

    0.694

    0.671

    0.682

    0.524

    9.4

    0.453

    0.443

    0.448

    0.289

    4.7

    0.278

    0.284

    0.281

    0.123

    0

    0.162

    0.155

    0.159

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.; (Microbial infection) Is unable to assume its function upon interaction with viral proteins, thereby increasing Fe concentration in the cytoplasm. This would inhibit the accumulation of reactive oxygen in host cells, leading to reduced apoptosis and increasing the survival of virus infected cell.
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Ferritin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: ssc:397030

    UniGene: Ssc.23940