Pig Follicle-stimulating hormone,FSH ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬促卵泡素(FSH)酶聯(lián)免疫試劑盒（CSB-E06791p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FSH含量。FSH（促卵泡激素）是一種由垂體分泌的激素，對生殖和發(fā)育至關(guān)重要。其研究機(jī)制涉及FSH與FSHR（促卵泡激素受體）的結(jié)合，通過G蛋白偶聯(lián)受體途徑調(diào)節(jié)細(xì)胞內(nèi)信號傳導(dǎo)，影響卵泡生長、雌激素生成和精子生成。試劑盒檢測范圍為20 mIU/mL-700 mIU/mL，可廣泛應(yīng)用于畜牧科研領(lǐng)域，如繁殖生理機(jī)制研究、生殖障礙模型分析、促排卵藥物效果評估以及種豬繁殖力篩選等場景，為動(dòng)物生殖健康研究提供可靠的數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

FSHBFollitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
縮寫：

FSH
Uniprot No.：

P01228
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

20 mIU/mL-700 mIU/mL
靈敏度：

10 mIU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	94
	Range %	90-98
1:4	Average %	92
	Range %	87-97
1:8	Average %	84
	Range %	80-88

回收率：

The recovery of pig FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	97	91-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
基因功能參考文獻(xiàn)：
1. As in vitro experiments in the present study suggested that LHX2 regulated the expression of Fshbeta, it is possible that LHX2 controls FSH synthesis at the transcriptional level. PMID: 22134063
2. Results showed that the effects of FSHb, ESR, and PRLR genes were significant in the Tibet pig population, and the effective genotypes of the three genes for reproductive traits were BB, BB, and AA, respectively. PMID: 20466637
3. LHX3 LIM homeodomain transcription factor is involved in activation of the FSH beta-subunit gene in the pituitary gonadotrope cell. PMID: 15271874
4. Prop-1 participates in the regulation of FSHbeta gene PMID: 15474519
5. A allele of FSHbeta-1 locus in all the population and FSHbeta-2 locus in pigs of the first parity had the positive effective on TNB, NBA, and WB. PMID: 18065386
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
數(shù)據(jù)庫鏈接：

KEGG: ssc:396895

STRING: 9823.ENSSSCP00000014160

UniGene: Ssc.5833