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  4. Pig Hepcidin(HAMP) ELISA kit

Pig Hepcidin(HAMP) ELISA kit

  • 中文名稱：
    豬鐵調(diào)素(HAMP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL010124PI
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬鐵調(diào)素(HAMP)酶聯(lián)免疫試劑盒（CSB-EL010124PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HAMP含量。試劑盒檢測范圍為9.38 ng/mL-600 ng/mL，可為豬鐵代謝機制研究、營養(yǎng)性缺鐵模型構(gòu)建、疾病狀態(tài)下鐵調(diào)素動態(tài)變化分析等科研項目提供可靠工具，尤其適用于農(nóng)業(yè)科學(xué)、獸醫(yī)學(xué)領(lǐng)域中對鐵穩(wěn)態(tài)調(diào)控的分子機制探索及生物標(biāo)志物篩選。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    HAMP ELISA Kit; Hepcidin ELISA Kit
  • 縮寫：
    HAMP
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    9.38 ng/mL-600 ng/mL
  • 靈敏度：
    2.34 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of pig HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    103

    Range %

    99-109

    1:2

    Average %

    94

    Range %

    89-99

    1:4

    Average %

    98

    Range %

    93-103

    1:8

    Average %

    89

    Range %

    84-95

  • 回收率：

    The recovery of pig HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    91-100

    EDTA plasma (n=4)

    99

    94-104

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    600

    2.751

    2.634

    2.692

    2.543

    300

    2.164

    2.267

    2.215

    2.066

    150

    1.424

    1.368

    1.396

    1.247

    75

    0.859

    0.829

    0.844

    0.695

    37.5

    0.428

    0.421

    0.424

    0.275

    18.75

    0.283

    0.273

    0.278

    0.129

    9.38

    0.201

    0.194

    0.198

    0.049

    0

    0.153

    0.145

    0.149

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.; Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
  • 基因功能參考文獻：
    1. this study demonstrates that urine hepcidin-25 concentrations strongly correlate with hepatic hepcidin mRNA abundance, plasma hepcidin-25 levels, iron transferrin saturation and non-heme liver iron levels. PMID: 26323096
    2. Data suugest that hepcidin might had antiinflammatory function and is a candidate regulator of the cross-talk between iron regulation and inflammation. PMID: 25774043
    3. report the full-length cDNA sequences of porcine hepcidin and liver-expressed antimicrobial peptide-2 (LEAP-2) PMID: 16051358
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Hepcidin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: ssc:397207

    STRING: 9823.ENSSSCP00000028422

    UniGene: Ssc.376