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  4. Pig Insulin,INS ELISA Kit

Pig Insulin,INS ELISA Kit

  • 中文名稱：
    豬胰島素(INS)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06828p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬胰島素(INS)酶聯(lián)免疫試劑盒（CSB-E06828p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的INS含量。INS為胰島素基因，其編碼的胰島素是調(diào)節(jié)血糖的關(guān)鍵激素。在研究機(jī)制上，它參與糖代謝過程，維持血糖平衡。胰島素分泌異常可能引發(fā)糖尿病等疾病，因此圍繞INS的功能、調(diào)控及相關(guān)治療靶點(diǎn)的研究，對(duì)代謝疾病防治意義重大。試劑盒檢測(cè)范圍為2 μIU/mL-400 μIU/mL，適用于糖尿病發(fā)病機(jī)制研究、胰島素分泌功能評(píng)估、代謝類藥物或飼料添加劑的藥效學(xué)分析，以及動(dòng)物實(shí)驗(yàn)中胰腺組織INS表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    2 μIU/mL-400 μIU/mL
  • 靈敏度：
    1 μIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 84-105  
    1:2 Average % 93  
    Range % 89-99  
    1:4 Average % 95  
    Range % 89-101  
    1:8 Average % 95  
    Range % 83-103  
  • 回收率：
    The recovery of pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-101  
    EDTA plasma (n=4) 98 92-104  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average    
    0 1.800 1.864 1.832    
    2 1.569 1.581 1.575    
    8 1.304 1.327 1.316    
    32 0.812 0.805 0.809    
    100 0.389 0.393 0.391    
    400 0.180 0.183 0.182    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 基因功能參考文獻(xiàn)：
    1. We expect that these insulin-deficient pigs can be used in diabetes research to test the efficacy and safety of new drugs and the recipient of islet transplantation to investigate optimal transplantation strategies. PMID: 29691708
    2. Study used well-tempered bias exchange metadynamics simulations to determine the equilibrium ensembles of an insulin molecule under amyloidogenic conditions of low pH and high temperature. The folded state of a single insulin molecule was shown to be the most stable, longest-lived state even under amyloidogenic conditions. PMID: 28445749
    3. The findings are consistent with previous studies that indicate a link between Na,K-ATPase activity and SFK signaling. PMID: 27748508
    4. PTPLAD1 and AMPK are rapidly compartmentalized within the plasma membrane (PM) and Golgi/endosome fractions after insulin stimulation and that ATIC later accumulates in the Golgi/endosome fraction. PMID: 25687571
    5. Pdx-1, MafA and NeuroD1 bind to the A, C and E elements in the insulin promoter and regulate the transcriptional activity of the insulin promoter. PMID: 24825179
    6. The interplay of the adiponectin system, TNFalpha and insulin at a transcriptional level and, their effects on the adipogenic transcription factor PPARgamma, as well as on the activation of main insulin signaling pathways, is reported. PMID: 24846393
    7. Thermodynamics of insulin unfolding have been quantified by differential scanning calorimetry and thermal unfolding measurements to determine the extent and nature of their stabilization of the insulin hexamer. PMID: 24811232
    8. Exposing the hydrophobic core of insulin can induce the increase of amyloidogenicity and formation of higher-order polymerized fibrils, which is less toxic to membranes. PMID: 24041697
    9. Data suggest that a mutation in INS (C94Y) results a transgenic disease model for the investigation of permanent neonatal diabetes. PMID: 23274907
    10. The results show that modulation of plasma insulin levels by dietary carbohydrates seems possible in anabolic sows, but IGF-I levels are less easily modified. PMID: 21635573
    11. insulin increased GCLc promoter activity, which required a prerequisite increase or decrease in medium glucose PMID: 21871559
    12. SOCS3 is an important negative regulator of insulin signaling in porcine adipocytes. PMID: 20683642
    13. Plasma concentrations of insulin in pigs fed once per day were lower before feeding than after the meal. Plasma concentrations of insulin in ad libitum fed pigs exhibited random fluctuations. PMID: 20399063
    14. Data show that an expression cassette containing 1500bp of the porcine insulin promoter 5' UTR confers robust and specific transgene expression to beta-cells in vitro and in transgenic mice. PMID: 19682540
    15. Neutron diffraction data for T(6) porcine insulin were collected to 2.1 A resolution from a single crystal partly deuterated by exchange of mother liquor. PMID: 19770501
    16. computational analysis of the altered ionization of the B13 Glu in insulin B9 and B10 mutants of 4INS PMID: 15326283
    17. Results describe the in vitro folding/unfolding of insulin/single-chain insulin. PMID: 16800793
    18. A two-stage in vitro folding pathway of insulin is proposed, with six major folding intermediates captured during the folding process. PMID: 17914645
    19. Streptozotocin is able to impair in vitro neonatal pancreatic beta cell insulin release whereas human cytokines is not. PMID: 17991146
    20. A16Leu had much more significant effects on the foldability of insulin than B17Leu. PMID: 18071885
    21. These data demonstrated that feed allowance level has important consequence on embryo survival, embryo development and uterine development, possibly mediated by changes in concentrations of reproductive hormones. PMID: 18700853
    22. Functional surfaces of insulin were probed by photocross-linking of an extensive set of azido derivatives constructed by chemical synthesis. PMID: 19321435

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: ssc:397415

    STRING: 9823.ENSSSCP00000025428

    UniGene: Ssc.583