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  4. Pig Interferon γ,IFN-γ ELISA Kit

Pig Interferon γ,IFN-γ ELISA Kit

  • 中文名稱：
    豬γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06794p-IS
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒（CSB-E06794p-IS）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IFNG含量。IFNG即干擾素-γ，是一種具有多種生物學(xué)功能的細(xì)胞因子。它在免疫調(diào)節(jié)中發(fā)揮核心作用，可增強(qiáng)巨噬細(xì)胞活性、促進(jìn)Th1細(xì)胞分化。研究機(jī)制上，通過與受體結(jié)合激活信號(hào)通路，調(diào)節(jié)免疫相關(guān)基因表達(dá)，在抗感染、抗腫瘤及自身免疫病研究中備受關(guān)注。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，靈敏度為3.12 pg/mL。該產(chǎn)品適用于動(dòng)物免疫學(xué)、傳染病模型研究、疫苗效力評(píng)估等科研領(lǐng)域，可定量分析豬感染性疾病（如豬繁殖與呼吸綜合征、豬瘟等）或炎癥反應(yīng)過程中IFN-γ的動(dòng)態(tài)變化，為研究宿主免疫應(yīng)答機(jī)制、病原體與宿主相互作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    3.12 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    101

    Range %

    94-108

    1:2

    Average %

    92

    Range %

    85-99

    1:4

    Average %

    96

    Range %

    91-101

    1:8

    Average %

    93

    Range %

    86-99

  • 回收率：

    The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    86-96

    EDTA plasma (n=4)

    92

    87-98

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    400

    2.687

    2.634

    2.660

    2.547

    200

    1.913

    1.967

    1.940

    1.827

    100

    1.233

    1.188

    1.211

    1.097

    50

    0.659

    0.629

    0.644

    0.531

    25

    0.358

    0.321

    0.340

    0.226

    12.5

    0.223

    0.233

    0.228

    0.115

    6.25

    0.176

    0.184

    0.180

    0.067

    0

    0.115

    0.111

    0.113

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 基因功能參考文獻(xiàn)：
    1. The regulatory effect of IFN-gamma on CYP3A29 expression is mediated via PXR. PMID: 25413352
    2. Mapping of QTL for mycoplasma and tetanus antibodies and IFNgamma. PMID: 20567833
    3. Data show taht the expression of PoIFN-gamma in insect cells was confirmed by Western Blot, indirect immunofluorescence assay and indirect ELISA. PMID: 17577980
    4. IFNgamma production from the frozen peripheral blood mononuclear cells was significantly higher than that from the fresh ones. PMID: 19766617
    5. Translational control of IL-18 expression by its 5'-UTR limits production of IL-18, resulting in restricted expression of mRNA and protein IFN-gamma in this model of crescentic glomerulonephritis(GN). Might amplify CD8+-mediated macrophage-dependent GN. PMID: 12787406
    6. selective changes in immature crypt cells induced by IFN-gamma bound to extracellular matrix could contribute to inappropriate responsiveness to commensal bacteria in inflammatory bowel diseases PMID: 15894119
    7. A functional single nucleotide polymorphism is reported in the coding region of IFN-gamma cDNA that markedly reduces antiviral activity of the IFN-gamma protein. PMID: 17416419
    8. The expression of IFN-gamma in recombiannt Lactococcus lactis as a tool for investigating downregulation of allergic predisposition of pigs to experimental food allergy is reported. PMID: 18164876

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 組織特異性：
    Released primarily from activated T lymphocytes.
  • 數(shù)據(jù)庫鏈接：