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  4. Pig Interferon β,IFN-β/IFNB ELISA Kit

Pig Interferon β,IFN-β/IFNB ELISA Kit

  • 中文名稱：
    豬β干擾素(IFN-β/IFNB)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09890p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬β干擾素(IFN-β/IFNB)酶聯(lián)免疫試劑盒（CSB-E09890p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的IFN-β/IFNB含量。IFN-β/IFNB即β-干擾素，它具有抗病毒、免疫調(diào)節(jié)等作用。在研究機制上，它能與細胞表面受體結(jié)合，激活相關(guān)信號通路，誘導(dǎo)抗病毒蛋白產(chǎn)生，從而抑制病毒復(fù)制。目前在治療多發(fā)性硬化癥等疾病方面有重要研究價值。試劑盒檢測范圍為50 pg/mL-2000 pg/mL，該產(chǎn)品適用于體外科研場景中豬IFN-β的動態(tài)水平監(jiān)測，如病毒感染后免疫應(yīng)答研究、炎癥模型評估或生物制劑開發(fā)過程中的效價分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IFN-β/IFNB
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    50 pg/mL-2000 pg/mL
  • 靈敏度：
    25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 85-86  
    1:2 Average % 97  
    Range % 92-101  
    1:4 Average % 89  
    Range % 84-93  
    1:8 Average % 95  
    Range % 90-99  
  • 回收率：
    The recovery of pig IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-101  
    EDTA plasma (n=4) 84 80-88  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 2.897 2.851 2.874    
    1000 2.089 2.072 2.081    
    500 1.196 1.163 1.180    
    250 0.649 0.687 0.668    
    100 0.286 0.293 0.290    
    50 0.144 0.146 0.145    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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