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  4. Pig Interleukin 18,IL-18 ELISA Kit

Pig Interleukin 18,IL-18 ELISA Kit

  • 中文名稱：
    豬白介素18(IL-18)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06781p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬白介素18(IL-18)酶聯(lián)免疫試劑盒（CSB-E06781p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL18含量。IL18 即白細(xì)胞介素 18，是一種多功能促炎細(xì)胞因子，在先天免疫和獲得性免疫中均發(fā)揮重要作用。其研究機(jī)制主要圍繞它能誘導(dǎo) T 細(xì)胞和自然殺傷細(xì)胞產(chǎn)生γ-干擾素，增強(qiáng)自然殺傷細(xì)胞的細(xì)胞毒活性，在炎癥、免疫相關(guān)疾病研究中備受關(guān)注。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于科研場(chǎng)景中動(dòng)態(tài)監(jiān)測(cè)實(shí)驗(yàn)動(dòng)物模型（如細(xì)菌/病毒感染、免疫調(diào)節(jié)研究）的免疫應(yīng)答水平；為獸醫(yī)學(xué)、比較免疫學(xué)及動(dòng)物疾病模型研究提供可靠工具，助力科研人員解析IL - 18在豬免疫系統(tǒng)中的分子機(jī)制與調(diào)控網(wǎng)絡(luò)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %88
    Range %82-92
    1:2Average %100
    Range %95-105
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of pig IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-93
    EDTA plasma (n=4)9491-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.562 2.687 2.625 2.443
    10001.839 1.865 1.852 1.670
    5001.104 1.135 1.120 0.938
    2500.643 0.638 0.641 0.459
    1250.412 0.435 0.424 0.242
    62.50.321 0.341 0.331 0.149
    31.250.262 0.248 0.255 0.073
    00.181 0.183 0.182
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
  • 基因功能參考文獻(xiàn)：
    1. Study reports that the monocyte-derived cytokines IL-12 and IL-18 act synergistically on porcine gammadelta T cells to induce IFN-gamma production. Additional stimuli such as the mitogen ConA and IL-2 are necessary to activate the cells for enhanced proliferation. PMID: 25036760
    2. IL-18 concentration in saliva was significantly increased during a 60-min acute immobilization stress in thirteen 5-month-old pigs. These results are the first evidence of a stress-related change of IL-18 in pig saliva. PMID: 21682650
    3. endometrial expression of CASP1 and IL18 associated with pregnancy establishment; alteration of CASP1 and IL18 following premature exposure of uterus to estrogen during early pregnancy may contribute to conceptus loss between Days 15 to 18 of pregnancy PMID: 20380728
    4. Porcine IL-18 regulates anti-pig cellular rejection in C57BL/6 mice. PMID: 17079874
  • 亞細(xì)胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ssc:397057

    STRING: 9823.ENSSSCP00000015962

    UniGene: Ssc.20