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  4. Pig Interleukin 1β,IL-1β ELISA Kit

Pig Interleukin 1β,IL-1β ELISA Kit

  • 中文名稱：
    豬白介素1β (IL-1β)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06782p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬白介素1β (IL-1β)酶聯(lián)免疫試劑盒（CSB-E06782p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL1B含量。IL1B，即白細胞介素-1β，是一種強效的促炎細胞因子，主要由單核細胞、巨噬細胞和樹突狀細胞產(chǎn)生。它在炎癥和免疫反應(yīng)中起關(guān)鍵作用，通過炎癥小體激活和蛋白水解過程產(chǎn)生活性形式，進而調(diào)節(jié)多種細胞活動，包括細胞增殖、分化和凋亡。試劑盒檢測范圍為3.9 pg/mL-250 pg/mL，該產(chǎn)品可廣泛應(yīng)用于動物醫(yī)學研究領(lǐng)域，包括但不限于感染性疾病模型的炎癥反應(yīng)評估、免疫調(diào)節(jié)藥物開發(fā)中的療效監(jiān)測，以及疫苗研發(fā)過程中細胞因子動態(tài)變化分析，為科研人員提供穩(wěn)定可靠的檢測平臺。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    3.9 pg/mL-250 pg/mL
  • 靈敏度：
    0.975 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-100
    1:2Average %96
    Range %91-103
    1:4Average %95
    Range %90-110
    1:8Average %95
    Range %85-105
  • 回收率：
    The recovery of pig IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9583-104
    EDTA plasma (n=4)9890-108
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2502.675 2.815 2.745 2.597
    1252.154 2.147 2.151 2.003
    62.51.312 1.335 1.324 1.176
    31.250.788 0.764 0.776 0.628
    15.60.377 0.356 0.367 0.219
    7.80.264 0.269 0.267 0.119
    3.90.190 0.188 0.189 0.041
    00.153 0.143 0.148 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：細胞培養(yǎng)上清液

樣品信息：其他

稀釋比：沒有稀釋

產(chǎn)品評價： 結(jié)果很滿意，也是對比了別家的試劑盒，最后發(fā)現(xiàn)還是華美的好用，我用的是細胞培養(yǎng)基上清，別家的試劑盒有干擾因素，華美的沒有，試了好幾家，也就這個牌子的好用。

By 高老師

相關(guān)問答

■ 常見問題解答
Q:

Hello, please advise if the Pig IL-1b ELISA kit (#CSB-E06782p) recognizes only mature IL-1b or both mature and pro-IL-1b protein. Thank you.

A:
Thanks for your inquiry. This kit detects total IL-1b, including mature form and pro form.

靶點詳情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 基因功能參考文獻：
    1. Analysis of host-protein interactions for African swine fever virus L83L identified IL-1beta as its host ligand. A recombinant African swine fever virus lacking the L83L was developed from the highly virulent field isolate Georgia2007 and was used to show that L83L is a non-essential gene. PMID: 29605728
    2. This study showed that the presence of embryos in oviducts on days 2-3 after mating may influence the oviductal expression of the members of the IL-1beta system, determining the action of IL-1beta, which may be considered to be the earliest sign of pregnancy in pigs. PMID: 28532837
    3. This study showed that classical swine fever virus and p7 protein induced IL-1beta secretion and that p7 protein was a short-lived protein degraded by the proteasome. PMID: 25146005
    4. local expression of IL-1beta and IL-8 in non-bacterial thrombotic endocarditis, Staphylococcus aureus infective endocarditis, animals with S. aureus sepsis without endocarditis and controls PMID: 23879680
    5. IL1B regulates expression of IL1R1 and IL1RAP and stimulates expression of PTGS1 and PTGS2 that are considered to be the most rate-limiting enzymes for endometrial synthesis of prostaglandins during the peri-implantation period of pregnancy in pigs. PMID: 22572995
    6. the results presented here strongly suggest IL-1beta as a key molecule guiding tissue remodelling events after myocardial infarction. PMID: 20678474

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  • 亞細胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫鏈接：