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Pig Interleukin 22(IL-22) ELISA Kit

文獻引用 (2) 說明書下載

產品詳情

產品描述：

豬白介素22(IL-22)酶聯(lián)免疫試劑盒（CSB-E15847p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-22含量。IL-22是重要靶點。它是一種細胞因子，參與免疫調節(jié)。其背景在于在多種疾病如炎癥性腸病、皮膚病等中表達異常。研究機制方面，主要通過與受體結合，激活下游信號通路，影響細胞增殖、分化及炎癥反應，為相關疾病治療提供方向。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，適用于動物免疫調控研究、病原感染后細胞因子表達分析、腸道屏障功能評估等科研方向，為探索豬類疾病模型、疫苗效果評價及營養(yǎng)干預研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
縮寫：

IL-22
Uniprot No.：

F1SH68
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-104
1:2	Average %	96
	Range %	89-100
1:4	Average %	95
	Range %	89-98
1:8	Average %	103
	Range %	95-107

回收率：

The recovery of pig IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	91-103
EDTA plasma (n=4)	101	95-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.