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  4. Pig Interleukin-23 subunit alpha(IL23A) ELISA kit

Pig Interleukin-23 subunit alpha(IL23A) ELISA kit

  • 中文名稱：
    豬白介素-23 α亞基(IL23A)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011638PI
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬白介素-23 α亞基(IL23A)酶聯(lián)免疫試劑盒（CSB-EL011638PI）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL23A含量。IL23A是重要的藥物靶點(diǎn)。IL-23由IL23A和IL-12p40亞基組成，在自身免疫疾病病理中發(fā)揮關(guān)鍵作用。研究機(jī)制主要聚焦于其信號(hào)通路，通過阻斷它與受體結(jié)合，可調(diào)節(jié)免疫反應(yīng)，為相關(guān)疾病如銀屑病等的治療提供方向。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于科研領(lǐng)域?qū)ωi免疫系統(tǒng)功能的研究，如感染性疾病模型建立、免疫調(diào)節(jié)機(jī)制解析或炎癥相關(guān)分子通路的探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL23A; SGRF; Interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; Interleukin-23 subunit p19; IL-23p19
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL23A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %102
    Range %97-107
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of pig IL23A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9490-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.549 2.598 2.574 2.404
    10001.789 1.748 1.769 1.599
    5001.024 1.042 1.033 0.863
    2500.603 0.632 0.618 0.448
    1250.410 0.416 0.413 0.243
    62.50.318 0.338 0.328 0.158
    31.250.254 0.265 0.260 0.090
    00.171 0.169 0.170
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 基因功能參考文獻(xiàn)：
    1. These results provide genetic information about both swine IL-23 the IL-23 receptor alpha (IL-23Ralpha) and IL-12 receptor beta1 (IL-12Rbeta1), which allows for better understanding of interleukin systems involved in pig immunity. PMID: 22032895
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: ssc:100155546

    UniGene: Ssc.56047