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  4. Pig Interleukin 6,IL-6 ELISA Kit

Pig Interleukin 6,IL-6 ELISA Kit

  • 中文名稱：
    豬白介素6(IL-6)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06786p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬白介素6(IL-6)酶聯(lián)免疫試劑盒（CSB-E06786p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL6含量。IL6是一種多功能細胞因子，在免疫、組織再生和新陳代謝中發(fā)揮重要作用。它通過經(jīng)典信號轉(zhuǎn)導(dǎo)和反式信號傳導(dǎo)影響多種細胞功能，參與炎癥、免疫反應(yīng)、急性期反應(yīng)和代謝調(diào)節(jié)。IL6在多種疾病中起關(guān)鍵作用，如炎癥性疾病、自身免疫性疾病和癌癥，因此成為藥物研發(fā)的重要靶點。試劑盒檢測范圍為1.25 pg/mL-80 pg/mL，科研人員可將其應(yīng)用于豬類疾病模型研究，如評估細菌或病毒感染后的免疫應(yīng)答動態(tài)，分析炎癥性疾病機制，或探究營養(yǎng)干預(yù)、疫苗效果等實驗條件下IL - 6的表達變化，為動物免疫學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.25 pg/mL-80 pg/mL
  • 靈敏度：
    0.31 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 86  
    Range % 80-90  
    1:2 Average % 95  
    Range % 91-100  
    1:4 Average % 97  
    Range % 95-103  
    1:8 Average % 90  
    Range % 85-95  
  • 回收率：
    The recovery of pig IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-95  
    EDTA plasma (n=4) 99 95-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    80 2.212 2.101 2.157 2.066  
    40 1.725 1.704 1.715 1.624  
    20 1.223 1.194 1.209 1.118  
    10 0.871 0.809 0.840 0.749  
    5 0.519 0.504 0.512 0.421  
    2.5 0.332 0.315 0.324 0.233  
    1.25 0.192 0.182 0.187 0.096  
    0 0.093 0.089 0.091 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

平均分:
5.0分 - 2 個評價

樣品類型：細胞培養(yǎng)上清液

樣品信息：其他

稀釋比：沒有稀釋

產(chǎn)品評價： 結(jié)果很滿意，也是對比了別家的試劑盒，最后發(fā)現(xiàn)還是華美的好用，我用的是細胞培養(yǎng)基上清，別家的試劑盒有干擾因素，華美的沒有，試了好幾家，也就這個牌子的好用。

By 高老師

樣品類型：血清

樣品信息：其他 豬

稀釋比：沒有稀釋

產(chǎn)品評價： 出于一直以來對華美產(chǎn)品的信任，這一次測定豬血清還是使用了華美的試劑盒，IL-6檢測不明顯，加大樣本量希望再看情況如何。

By 楊老師

相關(guān)問答

■ 常見問題解答
Q:

我有一份已經(jīng)在1500 g下離心15分鐘的豬血清樣本。但是，這個試劑盒CSB-E06786p的說明書建議以1000 g離心15分鐘。我想知道這是否會對結(jié)果產(chǎn)生影響。

A:
本手冊建議以1000g15 min離心。上述1500 g、15 min的離心機在理論上影響不大。建議您主要檢查是否發(fā)生了溶血，或者離心機是否在低溫下運行，因為離心過程中的溫度會隨著速度和時間的增加而升高。

靶點詳情

  • 最新研究進展：
    IL-6是一種重要的細胞因子，參與多種生物學(xué)過程的調(diào)節(jié)，如炎癥反應(yīng)、免疫應(yīng)答和細胞增殖等。最新研究發(fā)現(xiàn)，IL-6與COVID-19感染的嚴重程度密切相關(guān)，高水平的IL-6水平與COVID-19患者的死亡率升高有關(guān)。同時，IL-6受體抑制劑托珠單抗（Tocilizumab）可用于治療COVID-19患者的炎癥反應(yīng)和肺損傷。此外，研究還表明，IL-6在許多癌癥中發(fā)揮作用，可以作為一種治療靶點。
  • 功能：
    Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells.; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.
  • 基因功能參考文獻：
    1. IL-6 was low or undetectable in Boar seminal plasma. PMID: 22482797
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: ssc:399500

    STRING: 9823.ENSSSCP00000024026

    UniGene: Ssc.62