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  1. 首頁(yè)
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Pig Mucin-2,MUC2 ELISA Kit
  • 中文名稱：
    豬粘蛋白/粘液素2(MUC2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E15066p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬粘蛋白/粘液素2(MUC2)酶聯(lián)免疫試劑盒（CSB-E15066p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MUC2含量。MUC2是一種分泌型粘蛋白，主要在小腸和結(jié)腸的杯狀細(xì)胞中表達(dá)。它在維持腸道穩(wěn)態(tài)中發(fā)揮重要作用，其破壞與多種疾病和癌癥有關(guān)。MUC2的研究涉及其在腸道黏膜屏障損傷中的作用，以及其基因表達(dá)調(diào)控機(jī)制。試劑盒檢測(cè)范圍為7.8 ng/mL-500 ng/mL，該產(chǎn)品適用于豬腸道黏膜屏障功能研究、消化道疾病模型中的黏液分泌機(jī)制探索，以及營(yíng)養(yǎng)干預(yù)對(duì)腸道健康影響的評(píng)估等科研領(lǐng)域，尤其適合通過(guò)多類型樣本（如腸道組織勻漿與體液樣本）對(duì)比分析MUC2的動(dòng)態(tài)變化，為豬的腸道生理病理研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    MUC2
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    7.8 ng/mL-500 ng/mL
  • 靈敏度：
    1.95 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig mucin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %87-94
    1:2Average %97
    Range %92-101
    1:4Average %90
    Range %87-98
    1:8Average %92
    Range %85-95
  • 回收率：
    The recovery of pig mucin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9388-96
    EDTA plasma (n=4)9895-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    5001.976 2.121 2.049 1.885
    2501.303 1.210 1.257 1.093
    1250.774 0.784 0.779 0.615
    62.50.428 0.425 0.427 0.263
    31.250.296 0.274 0.285 0.121
    15.620.235 0.250 0.243 0.079
    7.80.208 0.207 0.208 0.044
    00.158 0.169 0.164
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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